| Photodynamic inactivation of Candida albicans by BAM-SiPc. | |
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MedLine Citation:
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PMID: 19298354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 micromol l(-1) BAM-SiPc with a total fluence of 12 J cm(-2) at a cell density of 10(7) cells ml(-1). A short incubation time of 5-15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells. |
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Authors:
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Cheung-Wai So; Paul W K Tsang; Pui-Chi Lo; C J Seneviratne; Lakshman P Samaranayake; Wing-Ping Fong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-26 |
Journal Detail:
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Title: Mycoses Volume: 53 ISSN: 1439-0507 ISO Abbreviation: Mycoses Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8805008 Medline TA: Mycoses Country: Germany |
Other Details:
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Languages: eng Pagination: 215-20 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antifungal Agents
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pharmacology* Candida albicans / chemistry, drug effects* Cell Membrane / drug effects Humans Indoles / pharmacology* Microbial Viability / drug effects Molecular Structure Organosilicon Compounds / pharmacology* Photosensitizing Agents / pharmacology* Reactive Oxygen Species / analysis Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/BAM-SiPc; 0/Indoles; 0/Organosilicon Compounds; 0/Photosensitizing Agents; 0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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