Document Detail


Photodynamic action inhibits compound 48/80-induced exocytosis in rat peritoneal mast cells.
MedLine Citation:
PMID:  11799908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photostimulation of sulfonated aluminum phthalocyanine (SALPC)-loaded mast cells (20,000 lux, 2 min) itself caused neither exocytosis nor [Ca2+]i increase in isolated rat peritoneal mast cells. This result is incompatible with that reported in other cell types such as pancreatic acinar cells. Stimulation with 50 micrograms/ml compound 48/80, a direct G-protein activator, induced massive exocytosis which was easily detectable under conventional microscope. The fluorescent granules stained with sulforhodamine B were found to be numerous on the perimetry of mast cells, confirming occurrence of exocytosis. The stimulation also increased [Ca2+]i and cell volume before initiation of exocytosis. Pretreatment of the cells with photodynamic action with 5 microM SALPC inhibited the compound 48/80-induced exocytosis, but the [Ca2+]i increase and the increase of cell volume were unaffected. NaN3 at 0.5 mM could relieve the photodynamic action-induced inhibition of exocytosis. These results indicate that, unlikely to other secretory or contractile cells, photodynamic action with SALPC does not directly affect exocytotic machinery but modulates some functional proteins involved in signal transduction process which may be posterior to G-protein activation in mast cells. Singlet oxygen may be involved in the photodynamic action-induced modulation. A possible target protein can be a protein in the cell membrane which binds with a protein of a granular membrane during the course of exocytosis.
Authors:
S Hashikura; Y Satoh; Z J Cui; Y Habara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of veterinary research     Volume:  49     ISSN:  0047-1917     ISO Abbreviation:  Jpn. J. Vet. Res.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2002-01-21     Completed Date:  2002-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376567     Medline TA:  Jpn J Vet Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  239-47     Citation Subset:  IM    
Affiliation:
Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. habara@vetmed.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Exocytosis / drug effects*
Fluorescent Dyes / diagnostic use
Indoles / pharmacology*
Light*
Male
Mast Cells / drug effects,  physiology*
Microscopy, Confocal
Organometallic Compounds / pharmacology*
Peritoneum / cytology
Photosensitizing Agents / pharmacology*
Rats
Rats, Sprague-Dawley
Rhodamines / diagnostic use
p-Methoxy-N-methylphenethylamine / pharmacology*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indoles; 0/Organometallic Compounds; 0/Photosensitizing Agents; 0/Rhodamines; 2609-88-3/lissamine rhodamine B; 4091-50-3/p-Methoxy-N-methylphenethylamine; 68637-19-4/aluminum phthalocyanine disulfonate; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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