| Photocurrent and Electronic Activities of Oriented-His-tagged Photosynthetic Light-Harvesting/Reaction Centre Core Complexes Assembled onto a Gold Electrode. | |
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MedLine Citation:
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PMID: 22239547 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A poly-histidine (His) tag was fused to the C- or N-terminus of the light-harvesting (LH1)-αchain of the photosynthetic antenna core complex (LH1-RC) from Rhodobacter sphaeroides to allow immobilization of the complex on a solid substrate with defined orientation. His-tagged LH1-RCs were adsorbed onto a gold electrode modified with Ni-NTA. The LH1-RC with the C-terminal His-tag (C-His LH1-RC) on the modified electrode produced a photovoltaic response upon illumination. Electron transfer is unidirectional within the RC, and starts when the bacteriochlorophyll a dimer in the RC is activated by light absorbed by LH1. The LH1-RC with the N-terminal His-tag (N-His LH1-RC) produced very little or no photocurrent upon illumination at any wavelength. The conductivity of the His-tagged LH1-RC was measured with point-contact current imaging atomic force microscopy, indicating that 60% of the C-His LH1-RC are corrected oriented (N-His 63%). The oriented C-His LH1-RC or N-His LH1-RC showed semi-conductive behavior i.e. had the opposite orientation. These results indicate that the His-tag successfully controlled the orientation of the RC on the solid substrate, and that the RC produced photocurrent depending upon the orientation on the electrode. |
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Authors:
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Masaharu Kondo; Kouji Iida; Takehisa Dewa; Hirofumi Tanaka; Takuji Ogawa; Sakiko Nagashima; Kenji V P Nagashima; Keizo Shimada; Hideki Hashimoto; Alastair T Gardiner; Richard J Cogdell; Mamoru Nango |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-12 |
Journal Detail:
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Title: Biomacromolecules Volume: - ISSN: 1526-4602 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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