| Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting. | |
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MedLine Citation:
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PMID: 20387987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bioprinting by the codeposition of cells and biomaterials is constrained by the availability of printable materials. Herein we describe a novel macromonomer, a new two-step photocrosslinking strategy, and the use of a simple rapid prototyping system to print a proof-of-concept tubular construct. First, we synthesized the methacrylated ethanolamide derivative of gelatin (GE-MA). Second, partial photochemical cocrosslinking of GE-MA with methacrylated hyaluronic acid (HA-MA) gave an extrudable gel-like fluid. Third, the new HA-MA:GE-MA hydrogels were biocompatible, supporting cell attachment and proliferation of HepG2 C3A, Int-407, and NIH 3T3 cells in vitro. Moreover, hydrogels injected subcutaneously in nude mice produced no inflammatory response. Fourth, using the Fab@Home printing system, we printed a tubular tissue construct. The partially crosslinked hydrogels were extruded from a syringe into a designed base layer, and irradiated again to create a firmer structure. The computer-driven protocol was iterated to complete a cellularized tubular construct with a cell-free core and a cell-free structural halo. Cells encapsulated within this printed construct were viable in culture, and gradually remodeled the synthetic extracellular matrix environment to a naturally secreted extracellular matrix. This two-step photocrosslinkable biomaterial addresses an unmet need for printable hydrogels useful in tissue engineering. |
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Authors:
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Aleksander Skardal; Jianxing Zhang; Lindsi McCoard; Xiaoyu Xu; Siam Oottamasathien; Glenn D Prestwich |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2011-01-25 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 2675-85 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of Utah, Salt Lake City, Utah, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomimetic Materials / chemical synthesis* Cell Culture Techniques / methods* Cell Proliferation Cell Separation / methods* Cell Survival Cross-Linking Reagents / chemical synthesis, radiation effects Extracellular Matrix / chemistry*, radiation effects Gelatin / chemical synthesis*, pharmacology, radiation effects Hyaluronic Acid / chemical synthesis*, pharmacology, radiation effects Hydrogels / chemical synthesis, pharmacology, radiation effects Light Materials Testing Mice Mice, Nude Micromanipulation / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DC004336-13/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cross-Linking Reagents; 0/Hydrogels; 9000-70-8/Gelatin; 9004-61-9/Hyaluronic Acid |
| Comments/Corrections | |
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