| Photochemical and phototoxic activity of berberine on murine fibroblast NIH-3T3 and Ehrlich ascites carcinoma cells. | |
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MedLine Citation:
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PMID: 16905326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study demonstrates photoinduced generation of superoxide anion radical and singlet oxygen upon UVA irradiation of berberine chloride, and its cytotoxic/phototoxic effects on murine fibroblast non-cancer NIH-3T3 and Ehrlich ascites carcinoma (EAC) cells. The EPR spectra monitored upon photoexcitation of aerated solutions of berberine evidenced the efficient activation of molecular oxygen via Type I and II mechanisms, as the generation of superoxide anion radical and singlet oxygen was observed. The EAC cell line was more sensitive to the effect of non-photoactivated and photoactivated berberine than the NIH-3T3 cell line. UVA irradiation increased the sensitivity of EAC cells to berberine, while the sensitivity of NIH-3T3 cells to photoactivated berberine was not changed. Berberine significantly induced direct DNA strand breaks in tested cells, oxidative lesions were not detected, and the effect of irradiation of cells after berberine treatment did not affect the increase of DNA damage in EAC and NIH-3T3 cells. The DNA damage generated by a combination of berberine with UVA irradiation induced a significant blockage of EAC cells in the S and G(2)/M phases and the stopping/decrease of cell proliferation after 24h of influence. On the other hand, after 36h or 48h of berberine treatment, the DNA damage induced necrotic or apoptotic death of EAC cells. Whether these divergences are caused by differences in the properties of two non-isogenic cell lines or by different berberine uptake and cell localization will be analyzed in our further investigations. |
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Authors:
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Sona Jantová; Silvia Letasiová; Vlasta Brezová; L'ubos Cipák; Juraj Lábaj |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-08-14 |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 85 ISSN: 1011-1344 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-10-23 Completed Date: 2007-02-12 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: Switzerland |
Other Details:
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Languages: eng Pagination: 163-76 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37 Bratislava, Slovak Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Berberine / pharmacology* Carcinoma, Ehrlich Tumor Cell Cycle / drug effects DNA / drug effects DNA Damage* Dose-Response Relationship, Drug Electron Spin Resonance Spectroscopy Humans Mice NIH 3T3 Cells Oxidants, Photochemical / pharmacology* Reactive Oxygen Species / adverse effects, chemical synthesis Spin Trapping Superoxides* Time Factors Tumor Cells, Cultured Ultraviolet Rays / adverse effects* |
| Chemical | |
Reg. No./Substance:
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0/Oxidants, Photochemical; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 2086-83-1/Berberine; 9007-49-2/DNA |
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