Document Detail


Photochemical Immobilization of Cells onto a Glass Substrate for <i>in situ</i> DNA Analysis.
MedLine Citation:
PMID:  22729038     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. In situ DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.
Authors:
Naoki Sasaki; Anri Isu; Reina Ishii; Kae Sato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  28     ISSN:  1348-2246     ISO Abbreviation:  Anal Sci     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  537-9     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University.
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