Document Detail


Photobody localization of phytochrome B is tightly correlated with prolonged and light-dependent inhibition of hypocotyl elongation in the dark.
MedLine Citation:
PMID:  24769533     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Photobody localization of Arabidopsis phytochrome B (phyB) fused to GFP (PBG) correlates closely with the photoinhibition of hypocotyl elongation. However, the N-terminal half of phyB fused to GFP (NGB) is hypersensitive to light despite its inability to localize to photobodies. Therefore, the significance of photobodies in regulating hypocotyl growth remains debatable. Accumulating evidence indicates that under diurnal conditions, photoactivated phyB persists into darkness to inhibit hypocotyl elongation. Here, we examine whether photobodies are involved in inhibiting hypocotyl growth in darkness by comparing the PBG and NGB lines after the red-light-to-dark transition. Surprisingly, after the transition from 10 μmol m-2 s-1 red light to darkness, PBG inhibits hypocotyl elongation three times longer than NGB. The disassembly of photobodies in PBG hypocotyl nuclei correlates tightly with the accumulation of the growth-promoting transcription factor PHYTOCHROME INTERACTING FACTOR 3 (PIF3). Destabilizing photobodies by either decreasing the light intensity or adding monochromatic far-red treatment before the light-to-dark transition leads to faster PIF3 accumulation and a dramatic reduction in the capacity for hypocotyl growth inhibition in PBG. In contrast, NGB is defective in PIF3 degradation and its hypocotyl growth in the dark is nearly unresponsive to changes in light conditions. Together, our results support the model that photobodies are required for the prolonged, light-dependent inhibition of hypocotyl elongation in the dark by repressing PIF3 accumulation and by stabilizing the Pfr form of phyB. Our study suggests that photobody localization patterns of phyB could serve as instructive cues that control light-dependent photomorphogenetic responses in the dark.
Authors:
Elise K Van Buskirk; Amit K Reddy; Akira Nagatani; Meng Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-25
Journal Detail:
Title:  Plant physiology     Volume:  -     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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