| Photobleaching of red fluorescence in oral biofilms. | |
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MedLine Citation:
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PMID: 21198645 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Hope CK, de Josselin de Jong E, Field MRT, Valappil SP, Higham SM. Photobleaching of red fluorescence in oral biofilms. J Periodont Res 2010; doi: 10.1111/j.1600-0765.2010.01334.x. © 2010 John Wiley & Sons A/S Background and Objective: Many species of oral bacteria can be induced to fluoresce due to the presence of endogenous porphyrins, a phenomenon that can be utilized to visualize and quantify dental plaque in the laboratory or clinical setting. However, an inevitable consequence of fluorescence is photobleaching, and the effects of this on longitudinal, quantitative analysis of dental plaque have yet to be ascertained. Material and Methods: Filter membrane biofilms were grown from salivary inocula or single species (Prevotella nigrescens and Prevotella intermedia). The mature biofilms were then examined in a custom-made lighting rig comprising 405 nm light-emitting diodes capable of delivering 220 W/m(2) at the sample, an appropriate filter and a digital camera; a set-up analogous to quantitative light-induced fluorescence digital. Longitudinal sets of images were captured and processed to assess the degradation in red fluorescence over time. Results: Photobleaching was observed in all instances. The highest rates of photobleaching were observed immediately after initiation of illumination, specifically during the first minute. Relative rates of photobleaching during the first minute of exposure were 19.17, 13.72 and 3.43 arbitrary units/min for P. nigrescens biofilms, microcosm biofilm and P. intermedia biofilms, respectively. Conclusion: Photobleaching could be problematic when making quantitative measurements of porphyrin fluorescence in situ. Reducing both light levels and exposure time, in combination with increased camera sensitivity, should be the default approach when undertaking analyses by quantitative light-induced fluorescence digital. |
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Authors:
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C K Hope; E de Josselin de Jong; M R T Field; S P Valappil; S M Higham |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-28 |
Journal Detail:
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Title: Journal of periodontal research Volume: - ISSN: 1600-0765 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2011-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0055107 Medline TA: J Periodontal Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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School of Dental Sciences Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool, UK Inspektor Research Systems BV, Amsterdam, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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