Document Detail

Photobiological effects of UVA and UVB light in zebrafish embryos: evidence for a competent photorepair system.
MedLine Citation:
PMID:  17716904     Owner:  NLM     Status:  MEDLINE    
The consequences of UVB and UVA irradiation on hatch rate, mortality, and malformation were studied in embryonic zebrafish (Danio rerio). The use of zebrafish embryos has expanded from traditional developmental models to diverse studies, including many techniques utilizing light exposure. To characterize useful indicators of photodamage, the responses and threshold limits of UV radiation as a function of embryonic stage and fish source were evaluated. Significant differences in UVB susceptibility were observed in embryos at 3, 6-7, 12, and 24h post-fertilization (hpf), with the 1000-cell stage (3 hpf) having greatest tolerance to UVB. Embryos derived from zebrafish raised in outdoor ponds were more tolerant to UVB than were embryos from laboratory-raised fish. Combinations of UVB and UVA exposure were used to confirm the presence of a competent photorepair system in zebrafish that could return otherwise malformed embryos to a normal phenotype. Overall, embryonic zebrafish had large tolerances (LD(50) of 850 J/cm(2)) to UVA, confirming their suitability for photoactivation and photorepair studies.
Qiaoxiang Dong; Kurt Svoboda; Terrence R Tiersch; W Todd Monroe
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-21
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  88     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  137-46     Citation Subset:  IM    
Department of Biological and Agricultural Engineering, 149 E.B. Doran Building, Louisiana State University and LSU AgCenter, Baton Rouge, LA 70803, USA.
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MeSH Terms
Dose-Response Relationship, Radiation
Embryo, Nonmammalian / metabolism,  radiation effects*,  ultrastructure
Embryonic Development / physiology,  radiation effects*
Gene Expression Regulation, Developmental / physiology,  radiation effects*
Time Factors
Ultraviolet Rays / adverse effects*
Zebrafish / embryology*,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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