Document Detail


Photobinding of 8-methoxypsoralen to transfer RNA and 5-fluorouracil-enriched transfer RNA.
MedLine Citation:
PMID:  343811     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The photobinding of [3H]8MOP to tRNA upon irradiation at 365 nm in the absence of O2 was determined by gel filtration. The maximum photobinding was found to be ca. 4 mol of 8MOP er mol of tRNA and 5FU-tRNA, with an overall quantum yield of 2.3 X 10(-3). The photobinding kinetics for 8MOP-tRNA showed an apparent induction period or sigmoidal kinetic curve, indicating a specific initial photobinding site on tRNA which was identified as 4-thiouridine at position 8 from the 5'-end of Escherichia coli tRNA. Photobinding of 8MOP to 5FU-tRNA proceeded without an apparent induction period. 8MOP-tRNA and 8MOP-5FU-tRNA adducts were characterized by absorption, fluorescence, and CD spectroscopy. A modified procedure was also developed to analyze the nucleoside composition in modified 8MOP-tRNA and 8MOP-5FU-tRNA. The results showed that 8MOP photochemically added mainly to pyrimidine bases. The photobinding of 8MOP changed the conformation (secondary in particular) of tRNA and inhibited aminoacyl-tRNA synthetase activity.
Authors:
C N Ou; P S Song
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  17     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-05-17     Completed Date:  1978-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1054-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acyl-tRNA Synthetases / metabolism
Circular Dichroism
Dose-Response Relationship, Radiation
Escherichia coli / enzymology
Fluorouracil*
Methoxsalen*
RNA, Transfer* / metabolism,  radiation effects
Spectrometry, Fluorescence
Transfer RNA Aminoacylation
Ultraviolet Rays
Chemical
Reg. No./Substance:
298-81-7/Methoxsalen; 51-21-8/Fluorouracil; 9014-25-9/RNA, Transfer; EC 6.1.1.-/Amino Acyl-tRNA Synthetases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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