Document Detail


Photoaging of human retinal pigment epithelium is accompanied by oxidative modifications of its eumelanin.
MedLine Citation:
PMID:  23421783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although photodegradation of the retinal pigment epithelium (RPE) melanin may contribute to the etiology of age-related macular degeneration, the molecular mechanisms of this phenomenon and the structural changes of the modified melanin remain unknown. Recently, we found that the ratio of pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) to pyrrole-2,3,5-tricarboxylic acid (PTCA) is a marker for the heat-induced cross-linking of eumelanin. In this study, we examined UVA-induced changes in synthetic eumelanins to confirm the usefulness of the PTeCA/PTCA ratio as an indicator of photo-oxidation and compared changes in various melanin markers and their ratios in human melanocytes exposed to UVA, in isolated bovine RPE melanosomes exposed to strong blue light and in human RPE cells from donors of various ages. The results indicate that the PTeCA/PTCA ratio is a sensitive marker for the oxidation of eumelanin exposed to UVA or blue light and that eumelanin and pheomelanin in human RPE cells undergo extensive structural modifications due to the life-long exposure to blue light.
Authors:
Shosuke Ito; Anna Pilat; Wolfram Gerwat; Christine M B Skumatz; Miho Ito; Atsumi Kiyono; Andrzej Zadlo; Yukiko Nakanishi; Ludger Kolbe; Janice M Burke; Tadeusz Sarna; Kazumasa Wakamatsu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-03-27
Journal Detail:
Title:  Pigment cell & melanoma research     Volume:  26     ISSN:  1755-148X     ISO Abbreviation:  Pigment Cell Melanoma Res     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-22     Completed Date:  2013-11-18     Revised Date:  2013-12-03    
Medline Journal Info:
Nlm Unique ID:  101318927     Medline TA:  Pigment Cell Melanoma Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  357-66     Citation Subset:  IM    
Copyright Information:
© 2013 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Aging / metabolism
Animals
Biological Markers / metabolism
Cattle
Child
Humans
Light*
Melanins / chemistry,  metabolism*
Melanocytes / metabolism,  radiation effects
Melanosomes / metabolism,  radiation effects
Middle Aged
Oxidation-Reduction / radiation effects
Photolysis / radiation effects*
Retinal Pigment Epithelium / radiation effects*
Tissue Donors
Ultraviolet Rays
Young Adult
Grant Support
ID/Acronym/Agency:
P30 EY001931/EY/NEI NIH HHS; P30EY01931/EY/NEI NIH HHS; R01EY013722/EY/NEI NIH HHS; R01EY019664/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Melanins; 0/pheomelanin; 12627-86-0/eumelanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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