| Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at position 148. | |
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MedLine Citation:
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PMID: 3864158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Irradiation of mixtures of diphtheria toxin fragment A and [carbonyl-14C]NAD with UV light (253.7 nm) is known to induce efficient transfer of the radiolabel to position 148, corresponding to glutamic acid in the unmodified protein. Here we report the structure of the photoproduct at position 148, as determined by chemical and photochemical methods, fast-atom-bombardment mass spectrometry, and nuclear magnetic resonance. The photoproduct [an alpha-amino-gamma-(6-nicotin-amidyl)butyric acid residue] contains the entire nicotinamide moiety of NAD linked via its number 6 carbon to the decarboxylated gamma-methylene carbon of Glu-148. No portion of the ADP-ribosyl group of NAD is present. These findings are consistent with the idea that Glu-148 lies at or near the catalytic center of diphtheria toxin. |
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Authors:
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S F Carroll; J A McCloskey; P F Crain; N J Oppenheimer; T M Marschner; R J Collier |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 82 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1985 Nov |
Date Detail:
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Created Date: 1985-12-05 Completed Date: 1985-12-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7237-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ADP Ribose Transferases* Affinity Labels Bacterial Toxins* Binding Sites Diphtheria Toxin / analysis*, radiation effects Exotoxins / analysis, radiation effects Magnetic Resonance Spectroscopy Mass Spectrometry NAD / analysis* Peptide Fragments / analysis*, radiation effects Photochemistry Protein Conformation Ultraviolet Rays Virulence Factors* |
| Grant Support | |
ID/Acronym/Agency:
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AI22021/AI/NIAID NIH HHS; CA39217/CA/NCI NIH HHS; GM22982/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Bacterial Toxins; 0/Diphtheria Toxin; 0/Exotoxins; 0/Peptide Fragments; 0/Virulence Factors; 0/diphtheria toxin fragment A; 53-84-9/NAD; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.31/toxA protein, Pseudomonas aeruginosa |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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