Document Detail


Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at position 148.
MedLine Citation:
PMID:  3864158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Irradiation of mixtures of diphtheria toxin fragment A and [carbonyl-14C]NAD with UV light (253.7 nm) is known to induce efficient transfer of the radiolabel to position 148, corresponding to glutamic acid in the unmodified protein. Here we report the structure of the photoproduct at position 148, as determined by chemical and photochemical methods, fast-atom-bombardment mass spectrometry, and nuclear magnetic resonance. The photoproduct [an alpha-amino-gamma-(6-nicotin-amidyl)butyric acid residue] contains the entire nicotinamide moiety of NAD linked via its number 6 carbon to the decarboxylated gamma-methylene carbon of Glu-148. No portion of the ADP-ribosyl group of NAD is present. These findings are consistent with the idea that Glu-148 lies at or near the catalytic center of diphtheria toxin.
Authors:
S F Carroll; J A McCloskey; P F Crain; N J Oppenheimer; T M Marschner; R J Collier
Related Documents :
15530988 - Synthesis of phosphonic analogues of carnitine and gamma-amino-beta-hydroxybutyric acid.
19058788 - Amino acid neurotransmitters assessed by proton magnetic resonance spectroscopy: relati...
6614448 - An improved method for the determination of gamma-carboxyglutamic acid in proteins, bon...
7435198 - Effect of gaba analogues on blood pressure and central gaba metabolism in the rat.
7792828 - Crystal structure of doisynolic acid and the structure of other products formed during ...
23435948 - Potent α-glucosidase and protein tyrosine phosphatase 1b inhibitors from artemisia capi...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1985-12-05     Completed Date:  1985-12-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7237-41     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
ADP Ribose Transferases*
Affinity Labels
Bacterial Toxins*
Binding Sites
Diphtheria Toxin / analysis*,  radiation effects
Exotoxins / analysis,  radiation effects
Magnetic Resonance Spectroscopy
Mass Spectrometry
NAD / analysis*
Peptide Fragments / analysis*,  radiation effects
Photochemistry
Protein Conformation
Ultraviolet Rays
Virulence Factors*
Grant Support
ID/Acronym/Agency:
AI22021/AI/NIAID NIH HHS; CA39217/CA/NCI NIH HHS; GM22982/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Affinity Labels; 0/Bacterial Toxins; 0/Diphtheria Toxin; 0/Exotoxins; 0/Peptide Fragments; 0/Virulence Factors; 0/diphtheria toxin fragment A; 53-84-9/NAD; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.31/toxA protein, Pseudomonas aeruginosa
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation and partial characterization of a Mr 32,000 protein with inhibin activity from porcine fol...
Next Document:  Expression of biologically active bovine luteinizing hormone in Chinese hamster ovary cells.