Document Detail


Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
MedLine Citation:
PMID:  23349004     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.
Authors:
Shota Morimoto; Takenori Tomohiro; Nobuyuki Maruyama; Yasumaru Hatanaka
Related Documents :
9174964 - Well defined dye adsorbents for protein purification.
23231504 - Moving to the core: spatiotemporal analysis of forkhead box o (foxo) and nuclear factor...
15196764 - Surface plasmon resonance immunosensor using self-assembled protein g for the detection...
10493174 - Molecular analysis of protein interactions mediating the function of the cell surface p...
22471784 - Minimalist probes for studying protein dynamics: thioamide quenching of selectively exc...
8380144 - Inhibition of protein phosphatases activates glucose-6-phosphatase in isolated rat hepa...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  -     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. ttomo@pha.u-toyama.ac.jp yasu@pha.u-toyama.ac.jp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Inhibin B Response in Male Rats Treated with a GnRH Agonist and an Endothelin Receptor Antagonis...
Next Document:  A high-confidence reference dataset of differentially expressed proteins in elongating cotton fiber ...