| Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein. | |
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MedLine Citation:
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PMID: 23349004 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites. |
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Authors:
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Shota Morimoto; Takenori Tomohiro; Nobuyuki Maruyama; Yasumaru Hatanaka |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-25 |
Journal Detail:
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Title: Chemical communications (Cambridge, England) Volume: - ISSN: 1364-548X ISO Abbreviation: Chem. Commun. (Camb.) Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9610838 Medline TA: Chem Commun (Camb) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. ttomo@pha.u-toyama.ac.jp yasu@pha.u-toyama.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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