| Photoactivation of the CreER T2 recombinase for conditional site-specific recombination with high spatiotemporal resolution. | |
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MedLine Citation:
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PMID: 20441524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales). |
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Authors:
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Deepak Kumar Sinha; Pierre Neveu; Nathalie Gagey; Isabelle Aujard; Thomas Le Saux; Christine Rampon; Carole Gauron; Koichi Kawakami; Christoph Leucht; Laure Bally-Cuif; Michel Volovitch; David Bensimon; Ludovic Jullien; Sophie Vriz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zebrafish Volume: 7 ISSN: 1557-8542 ISO Abbreviation: Zebrafish Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-10 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101225070 Medline TA: Zebrafish Country: United States |
Other Details:
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Languages: eng Pagination: 199-204 Citation Subset: IM |
Affiliation:
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Ecole Normale Supérieure, Laboratoire de Physique Statistique, UMR 8550 CNRS, Paris Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified DNA Primers / genetics Gene Expression Regulation, Enzymologic / physiology* HSP70 Heat-Shock Proteins / metabolism Integrases / metabolism* Microscopy, Fluorescence Photochemical Processes Polymerase Chain Reaction Recombinant Fusion Proteins / metabolism* Recombination, Genetic / physiology* Spectrometry, Fluorescence Zebrafish* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/HSP70 Heat-Shock Proteins; 0/Recombinant Fusion Proteins; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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