Document Detail

Photo-induced chemiluminescent method for determination of reducing sugars.
MedLine Citation:
PMID:  18452135     Owner:  NLM     Status:  MEDLINE    
In the present study, a simple and sensitive photo-induced chemiluminescence (CL) method for the quantitation of reducing sugars, including fructose, glucose, sucrose and lactose, was developed. This method was based on the on-line photocatalytic reaction of the reducing sugars, using a home-made photoreactor consisting of PTEF tube helically coiling around a high-pressure mercury UV lamp. Reducing sugars were detected by direct CL emission resulting from the reaction between the photoproducts and luminol. To maximize the signal intensity, the effects of irradiation time, flow rate, luminol concentration, buffer pH and concentration were tested. Under optimum conditions, the linear dynamic ranges were all 0.36-18 mg/L and the relative standard deviations (RSDs) were 1.8-2.3%, with limits of detection (3sigma) of 0.06 mg/L for fructose, glucose, sucrose and lactose. Finally, interference effects from ascorbic acid, amino acids (alanine, glycine, serine, leucine and methionine) and some metal ions and anions were also investigated.
He Chen; Ying Gao; Yingying Su; Yi Lv
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  23     ISSN:  1522-7243     ISO Abbreviation:  Luminescence     Publication Date:    2008 Sep-Oct
Date Detail:
Created Date:  2008-09-24     Completed Date:  2008-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  England    
Other Details:
Languages:  eng     Pagination:  287-91     Citation Subset:  IM    
Key Laboratory of Green Chemistry and Technology, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, People's Republic of China.
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MeSH Terms
Carbohydrates / analysis*
Chemiluminescent Measurements / methods*,  standards
Equipment Design
Fructose / analysis
Glucose / analysis
Lactose / analysis
Sucrose / analysis
Reg. No./Substance:
0/Carbohydrates; 30237-26-4/Fructose; 50-99-7/Glucose; 521-31-3/Luminol; 57-50-1/Sucrose; 63-42-3/Lactose

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