| Photo-cross-linking of type I collagen gels in the presence of smooth muscle cells: mechanical properties, cell viability, and function. | |
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MedLine Citation:
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PMID: 12857069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effectiveness of photomediated cross-linking of type I collagen gels in the presence of rat aortic smooth muscle cells (RASMC) as a method to enhance gel mechanical properties while retaining native collagen triple helical structure and maintaining high cell viability was investigated. Collagen was chemically modified to incorporate an acrylate moiety. Collagen methacrylamide was cast into gels in the presence of a photoinitiator along with RASMC. The gels were cross-linked using visible light irradiation. Neither acrylate modification nor the cross-linking reaction altered collagen triple helical content. The cross-linking reaction, however, moved the denaturation temperature beyond the physiologic range. A twelve-fold increase in shear modulus was observed after cross-linking. Cell viability in the range of 70% (n = 4, p > 0.05) was observed in the photo-cross-linked gels. Moreover the cells were able to contract the cross-linked gel in a manner commensurate with that observed for natural type I collagen. Methacrylate-mediated photo-cross-linking is a facile route to improve mechanical properties of collagen gels in the presence of cells while maintaining high cell viability. This enhances the potential for type I collagen gels to be used as scaffolds for tissue engineering. |
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Authors:
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William T Brinkman; Karthik Nagapudi; Benjamin S Thomas; Elliot L Chaikof |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomacromolecules Volume: 4 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2003 Jul-Aug |
Date Detail:
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Created Date: 2003-07-14 Completed Date: 2004-08-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 890-5 Citation Subset: IM |
Affiliation:
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Departments of Surgery and Biomedical Engineering, Emory University School of Medicine, Atlanta, Georgia 30322, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylamides
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metabolism Animals Aorta / cytology* Cell Survival / radiation effects Circular Dichroism Collagen Type I / chemistry*, radiation effects Cross-Linking Reagents Gels / chemistry* Muscle, Smooth, Vascular / cytology*, metabolism*, radiation effects Photochemistry / methods* Protein Denaturation Rats Rats, Sprague-Dawley Rheology Temperature Tissue Engineering / methods* |
| Chemical | |
Reg. No./Substance:
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0/Acrylamides; 0/Collagen Type I; 0/Cross-Linking Reagents; 0/Gels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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