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Photo-Induced Monolayer Patterning for the Creation of Complex Protein Patterns.
MedLine Citation:
PMID:  23145751     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This work investigates self-assembled monolayers that were formed from a glycol-terminated thiol monomer and were patterned using photo-induced monolayer desorption. Utilizing direct-write photolithography provided a facile means to generate complex protein patterns containing gradients and punctate regions. The ablated glycol monolayers were characterized using scanning probe microscopy, which allowed us to observe differences in the nanomechanical properties between the patterned and nonpatterned regions of the substrate. The patterned regions on the surface adsorbed proteins and this process was monitored quantitatively using surface plasma resonance imaging (SPRi). Moreover, the concentration of the protein could be controlled accurately by simply setting the gray-level in an 8-bit image. Adsorbed protein was probed using a commercially available antibody binding assay, which showed significant enhancement over background. The ability to produce complex protein patterns will contribute greatly to creating in vitro models that more accurately mimic an in vivo cellular environment.
Authors:
Matthew J Hynes; Joshua A Maurer
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-12
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  -     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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