| Phosphorylcholine stimulates capsule formation of phosphate-limited mucoid Pseudomonas aeruginosa. | |
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MedLine Citation:
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PMID: 3126146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Production of both alginic acid and lipopolysaccharide by a mucoid strain of Pseudomonas aeruginosa, SRM-3, was studied in a chemostat system during growth under nutrient-limiting conditions chosen to reflect the chronic growth conditions in the lungs of cystic fibrosis patients. Since mucoid strains have been shown to elaborate extracellular proteases and phospholipase C, nitrogen and phosphate limitation were selected for analysis. A modified alginate-promoting medium containing either 1 mM glutamate or 0.05 mM K2HPO4 as limiting nutrient and doubling times of 1.6 to 15.7 h were used. Under nitrogen limitation, strain SRM-3 produced 1.4 mg of uronic acid per mg (dry weight) of cells at all doubling times studied. However, phosphate limitation resulted in the synthesis of only 0.4 mg of uronic acid per mg (dry weight) of cells. The role of phosphate in alginic acid polysaccharide production was further investigated by using phosphorylcholine, a product of phospholipase C activity on phosphatidylcholine, the major lung surfactant. No only were mucoid cells capable of utilizing phosphorylcholine for growth, but a highly specific interaction occurred among phosphorylcholine, alginate, and whole cells, resulting in greatly enhanced culture viscosity. Electron micrographs showed the gradual formation of a capsule during growth on phosphorylcholine, indicating that the mucoid strain has the ability to utilize surfactant not only as a nutrient source but also for constructing a capsule with greatly enhanced adhesive properties. |
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Authors:
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D P Krieg; J A Bass; S J Mattingly |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 56 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1988 Apr |
Date Detail:
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Created Date: 1988-04-19 Completed Date: 1988-04-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 864-73 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alginates
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biosynthesis Choline / analogs & derivatives* Cystic Fibrosis / microbiology Extracellular Space / metabolism Glucuronic Acid Hexuronic Acids Microscopy, Electron Models, Biological Nitrogen / metabolism Phosphates / metabolism Phosphorylcholine / pharmacology* Polysaccharides, Bacterial / biosynthesis Pseudomonas aeruginosa / physiology*, ultrastructure Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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T32 AI 07271/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alginates; 0/Hexuronic Acids; 0/Phosphates; 0/Polysaccharides, Bacterial; 107-73-3/Phosphorylcholine; 576-37-4/Glucuronic Acid; 62-49-7/Choline; 7727-37-9/Nitrogen; 9005-32-7/alginic acid |
| Comments/Corrections | |
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