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Phosphorylation and ubiquitination of Dynamin related proteins (AtDRP3A/3B) synergically regulate mitochondrial proliferation during mitosis.
MedLine Citation:
PMID:  22595081     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The balance between mitochondrial fission and fusion is disrupted during mitosis, but the mechanism governing this phenomenon in plant cells remains enigmatic. Here, we used mitochondrial matrix-localized Kaede (mt-Kaede) to analyze the dynamics of mitochondrial fission in BY-2 suspension cells. Analysis of the photoactivatable fluorescence of mt-Kaede suggested that the fission process is dominant during mitosis. This finding was confirmed by an electron microscopic analysis of the size distribution of mitochondria in BY-2 suspension cells at different stages. Cellular proteins interacting with Myc-tagged dynamin related protein 3A/3B (AtDRP3A and AtDRP3B) were immunoprecipitated with anti-Myc antibody-conjugated beads and subsequently identified by microcapillary liquid chromatography-quadrupole time-of-flight mass spectrometry (CapLC Q-TOF) MS/MS. The identified proteins functions were broadly associated with cytoskeletal (microtubular), phosphorylation, or ubiquitination functions. Mitotic phosphorylation of AtDRP3A/AtDRP3B and mitochondrial fission at metaphase were inhibited by treatment of the cells with a CdkB/cyclin B inhibitor or a serine/threonine protein kinase inhibitor. The fate of AtDRP3A/3B during the cell cycle was followed by time-lapse imaging of the fluorescence of Dendra2-tagged AtDRP3A/3B after green-to-red photoconversion; this experiment showed that AtDRP3A/3B is partially degraded during interphase. Additionally, we found that microtubules are involved in mitochondrial fission during mitosis and that the actin cytoskeleton defines mitochondrial inheritance as early as metaphase. Taken together, these findings suggest that mitotic phosphorylation of AtDRP3A/3B promotes mitochondrial fission during plant cell mitosis and that AtDRP3A/3B is partially degraded at interphase, providing mechanistic insight into the mitochondrial morphologic changes associated with cell cycle transitions in BY-2 suspension cells. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Authors:
Feng Wang; Peng Liu; Quan Zhang; Jian Zhu; Tong Chen; Shin-Ichi Arimura; Nobuhiro Tsutsumi; Jinxing Lin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-17
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
Institute of Botany, Chinese Academy of Sciences, Key Laboratory of Plant Molecular Physiology, Beijing 100093, China Graduate School of Chinese Academy of Sciences, Beijing 100049, China Key Laboratory of Ministry of Education for Cell Proliferation and Differentiation, College of Life Sciences, Peking University, Beijing 100871, China School of Life Sciences and Biomedical Engineering, Tongji University, Shanghai 200331, China Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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