Document Detail


Phosphorylation of talin at tyrosine in Rous sarcoma virus-transformed cells.
MedLine Citation:
PMID:  3031468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytoskeletal protein talin was found to undergo enhanced phosphorylation at tyrosine residues in chicken embryo fibroblasts following transformation by Rous sarcoma virus. An increase in the tyrosine phosphorylation of talin was also observed within 6 h in cells infected by the temperature-sensitive mutant tsNY68 after a shift from the nonpermissive to the permissive temperature. The overall extent of phosphorylation was 0.07 mol of phosphate per mol of talin and was not appreciably altered by transformation. In uninfected cells talin was shown to be phosphorylated at multiple sites by tryptic peptide mapping. Following transformation most of these sites remained phosphorylated, to the same or to a lesser extent, while novel, phosphotyrosine-containing phosphopeptides appeared. Talin was phosphorylated at tyrosine in cells infected by Rous sarcoma virus mutants which induce altered or partial transformation morphologies; thus the increased phosphorylation of talin at tyrosine occurred irrespective of the morphology induced. Transformation by Y73 also induced elevated levels of phosphotyrosine in talin, whereas transformation by the avian erythroblastosis and Fujinami sarcoma viruses did not.
Authors:
J E DeClue; G S Martin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  7     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-04-27     Completed Date:  1987-04-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  371-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Avian Sarcoma Viruses / genetics*
Cell Transformation, Neoplastic*
Chick Embryo
Cytoskeletal Proteins / metabolism*
Fibroblasts / metabolism
Mutation
Peptide Mapping
Phosphorus Radioisotopes
Phosphorylation
Talin
Trypsin
Tyrosine / metabolism*
Grant Support
ID/Acronym/Agency:
17542//PHS HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cytoskeletal Proteins; 0/Phosphorus Radioisotopes; 0/Talin; 55520-40-6/Tyrosine; EC 3.4.21.4/Trypsin
Comments/Corrections

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