Document Detail


Phosphorylation profiles of 60 kD Ro antigen in synchronized HEp-2 cells.
MedLine Citation:
PMID:  8533044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine whether phosphorylation has an effect on the characteristics of the 60 kD Ro antigen throughout the cell cycle. Cell extracts of synchronized HEp-2 cells were phosphorylated in vitro with exogenous ATP, examined by SDS-PAGE and Western blot, and probed with specific anti-Ro sera. In addition, cellular ATP pools were radiolabelled in vivo with 32P. The presence of the Ro protein was detected with a molecular weight of 60 kD during all phases of the cell cycle, except at the M phase, where it was increased to 65 kD. Phosphorylation of the in vitro and in vivo cell extracts increased the molecular mass to 65 kD. Moreover immunoprecipitation assays demonstrated that Ro is hyperphosphorylated in the M phase. Phosphorylation did not change the recognition pattern of the anti-Ro sera.
Authors:
A López-Luna; R M Ramírez-Santoyo; O Y Barbosa-Cisneros; E Avalos-Díaz; J Moreno; R Herrera-Esparza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of rheumatology     Volume:  24     ISSN:  0300-9742     ISO Abbreviation:  Scand. J. Rheumatol.     Publication Date:  1995  
Date Detail:
Created Date:  1996-02-01     Completed Date:  1996-02-01     Revised Date:  2007-05-11    
Medline Journal Info:
Nlm Unique ID:  0321213     Medline TA:  Scand J Rheumatol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  293-9     Citation Subset:  IM    
Affiliation:
Department of Immunology, Universidad Autónoma de Zacatecas, Guadalupe, México.
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Autoantigens / chemistry,  metabolism*
Autoimmunity
Cell Cycle
Cell Line
Immune Sera / immunology
Mitosis
Molecular Weight
Phosphorylation
RNA, Small Cytoplasmic*
Ribonucleoproteins / chemistry,  metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Immune Sera; 0/RNA, Small Cytoplasmic; 0/Ribonucleoproteins; 0/SS-A antigen; 0/TROVE2 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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