| Phosphorylation networks regulating JNK activity in diverse genetic backgrounds. | |
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MedLine Citation:
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PMID: 18927396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cellular signaling networks have evolved to enable swift and accurate responses, even in the face of genetic or environmental perturbation. Thus, genetic screens may not identify all the genes that regulate different biological processes. Moreover, although classical screening approaches have succeeded in providing parts lists of the essential components of signaling networks, they typically do not provide much insight into the hierarchical and functional relations that exist among these components. We describe a high-throughput screen in which we used RNA interference to systematically inhibit two genes simultaneously in 17,724 combinations to identify regulators of Drosophila JUN NH(2)-terminal kinase (JNK). Using both genetic and phosphoproteomics data, we then implemented an integrative network algorithm to construct a JNK phosphorylation network, which provides structural and mechanistic insights into the systems architecture of JNK signaling. |
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Authors:
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Chris Bakal; Rune Linding; Flora Llense; Elleard Heffern; Enrique Martin-Blanco; Tony Pawson; Norbert Perrimon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 322 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-17 Completed Date: 2008-11-03 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 453-6 Citation Subset: IM |
Affiliation:
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Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02215, USA. cbakal@receptor.med.harvard.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Cell Line Computational Biology Drosophila / enzymology*, genetics Drosophila Proteins / genetics, metabolism* Fluorescence Resonance Energy Transfer Genes, Insect* JNK Mitogen-Activated Protein Kinases / genetics, metabolism* MAP Kinase Signaling System* Metabolic Networks and Pathways Phosphorylation Proteomics RNA Interference Signal Transduction |
| Grant Support | |
ID/Acronym/Agency:
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//Howard Hughes Medical Institute; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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