| Phosphorylation of histone H3 at serine 10 is indispensable for neoplastic cell transformation. | |
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MedLine Citation:
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PMID: 15994958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Very little is known about the role of histone H3 phosphorylation in malignant transformation and cancer development. Here, we examine the function of H3 phosphorylation in cell transformation in vivo. Introduction of small interfering RNA-H3 into JB6 cells resulted in decreased epidermal growth factor (EGF)-induced cell transformation. In contrast, wild-type histone H3 (H3 WT)-overexpressing cells markedly stimulated EGF-induced cell transformation, whereas the H3 mutant S10A cells suppressed transformation. When H3 WT was overexpressed, EGF induction of c-fos and c-jun promoter activity was significantly increased compared with control cells but not in the H3 mutant S10A or S28A cells. In addition, activator protein-1 activity in H3 WT-overexpressing cells was markedly up-regulated by EGF in contrast to the H3 mutant S10A or S28A cells. These results indicate that the phosphorylation of histone H3 at Ser10 is an essential regulatory mechanism for EGF-induced neoplastic cell transformation. |
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Authors:
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Hong Seok Choi; Bu Young Choi; Yong-Yeon Cho; Hideya Mizuno; Bong Seok Kang; Ann M Bode; Zigang Dong |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 65 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-04 Completed Date: 2005-09-07 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: United States |
Other Details:
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Languages: eng Pagination: 5818-27 Citation Subset: IM |
Affiliation:
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Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Transformation, Neoplastic / drug effects, genetics, metabolism* Epidermal Growth Factor / pharmacology Epithelial Cells / metabolism, physiology Gene Expression Regulation Genes, fos / genetics Genes, jun / genetics Histones / antagonists & inhibitors, genetics, metabolism* Mice Molecular Sequence Data Phosphorylation Promoter Regions, Genetic Proto-Oncogene Proteins c-fos / antagonists & inhibitors, biosynthesis, genetics, physiology Proto-Oncogene Proteins c-jun / antagonists & inhibitors, biosynthesis, genetics, physiology RNA, Messenger / genetics RNA, Small Interfering / genetics Serine / metabolism Transcription Factor AP-1 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA77646/CA/NCI NIH HHS; CA81064/CA/NCI NIH HHS; R01 CA077646-05/CA/NCI NIH HHS; R01 CA081064-06/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Histones; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/Transcription Factor AP-1; 56-45-1/Serine; 62229-50-9/Epidermal Growth Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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