| Phosphorylation of gamma-tubulin regulates microtubule organization in budding yeast. | |
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MedLine Citation:
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PMID: 11709183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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gamma-Tubulin is essential for microtubule nucleation in yeast and other organisms; whether this protein is regulated in vivo has not been explored. We show that the budding yeast gamma-tubulin (Tub4p) is phosphorylated in vivo. Hyperphosphorylated Tub4p isoforms are restricted to G1. A conserved tyrosine near the carboxy terminus (Tyr445) is required for phosphorylation in vivo. A point mutation, Tyr445 to Asp, causes cells to arrest prior to anaphase. The frequency of new microtubules appearing in the SPB region and the number of microtubules are increased in tub4-Y445D cells, suggesting this mutation promotes microtubule assembly. These data suggest that modification of gamma-tubulin is important for controlling microtubule number, thereby influencing microtubule organization and function during the yeast cell cycle. |
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Authors:
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J Vogel; B Drapkin; J Oomen; D Beach; K Bloom; M Snyder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental cell Volume: 1 ISSN: 1534-5807 ISO Abbreviation: Dev. Cell Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-15 Completed Date: 2002-01-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 621-31 Citation Subset: IM |
Affiliation:
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Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Anaphase Animals Benomyl / pharmacology Cell Cycle Drug Resistance, Fungal Electrophoresis, Gel, Two-Dimensional Genes, Dominant Microscopy, Fluorescence Microscopy, Video Microtubules / chemistry, metabolism* Mitotic Spindle Apparatus / chemistry, metabolism Molecular Sequence Data Mutagenesis, Site-Directed Phosphorylation Point Mutation Protein Isoforms Saccharomyces cerevisiae / cytology*, drug effects, genetics, metabolism* Saccharomyces cerevisiae Proteins* Sequence Homology, Amino Acid Time Factors Tubulin / chemistry, genetics, metabolism* Tyrosine / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM36494/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protein Isoforms; 0/Saccharomyces cerevisiae Proteins; 0/TUB4 protein, S cerevisiae; 0/Tubulin; 17804-35-2/Benomyl; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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