Document Detail

Phosphorylation of Hsl1 by Hog1 leads to a G2 arrest essential for cell survival at high osmolarity.
MedLine Citation:
PMID:  16688223     Owner:  NLM     Status:  MEDLINE    
Control of cell cycle progression by stress-activated protein kinases (SAPKs) is essential for cell adaptation to extracellular stimuli. Exposure of yeast to osmostress leads to activation of the Hog1 SAPK, which controls cell cycle at G1 by the targeting of Sic1. Here, we show that survival to osmostress also requires regulation of G2 progression. Activated Hog1 interacts and directly phosphorylates a residue within the Hsl7-docking site of the Hsl1 checkpoint kinase, which results in delocalization of Hsl7 from the septin ring and leads to Swe1 accumulation. Upon Hog1 activation, cells containing a nonphosphorylatable Hsl1 by Hog1 are unable to promote Hsl7 delocalization, fail to arrest at G2 and become sensitive to osmostress. Together, we present a novel mechanism that regulates the Hsl1-Hsl7 complex to integrate stress signals to mediate cell cycle arrest and, demonstrate that a single MAPK coordinately modulates different cell cycle checkpoints to improve cell survival upon stress.
Josep Clotet; Xavier Escoté; Miquel Angel Adrover; Gilad Yaakov; Eloi Garí; Martí Aldea; Eulàlia de Nadal; Francesc Posas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-11
Journal Detail:
Title:  The EMBO journal     Volume:  25     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-07     Completed Date:  2006-08-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  2338-46     Citation Subset:  IM    
Department of Molecular and Cellular Biology, Universitat Internacional de Catalunya, Sant Cugat del Vallès, Spain.
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MeSH Terms
Cell Cycle Proteins / genetics,  metabolism
Cell Survival*
Enzyme Activation
Enzyme Stability
G2 Phase / physiology*
Mitogen-Activated Protein Kinases / genetics,  metabolism*
Osmolar Concentration
Protein Kinases / genetics,  metabolism*
Protein-Arginine N-Methyltransferases
Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases / genetics,  metabolism
Saccharomyces cerevisiae / cytology,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Two-Hybrid System Techniques
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.1.1.-/Protein-Arginine N-Methyltransferases; EC protein, S cerevisiae; EC 2.7.-/Protein Kinases; EC 2.7.1.-/SWE1 protein, S cerevisiae; EC 2.7.10.-/HOG1 protein, S cerevisiae; EC Kinases; EC protein, S cerevisiae; EC Kinases; EC Protein Kinases

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