Document Detail


Phosphorylation of Chromatin-associated Proteins in Lemna and Hordeum.
MedLine Citation:
PMID:  16659068     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Sterile embryos of barley (Hordeum vulgare) and cultures of Lemna perpusilla have been labeled with (32)Pi and the chromatin proteins prepared and separated by acid-urea and sodium dodecyl sulfate gel electrophoresis. Under these conditions chromatin proteins became labeled and the gel radioactivity profiles which were complex indicated a probable minimum of 15 to 20 proteins phosphorylated with molecular weights ranging from 10(4) to 10(5). The majority of the radioactivity, 80 to 90% of the total, is found in the acidic protein fraction and this can be recovered as serine phosphate after partial acid hydrolysis.Nuclei have been isolated from Lemna and barley and found to possess endogenous kinase activity. In vitro labeling of these nuclei with (32)P-adenosine triphosphate indicated that similar proteins appear to become labeled as in vivo labeling with (32)Pi but the proportions of label in each protein were different.
Authors:
L C van Loon; A Trewavas; K S Chapman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  55     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-92     Citation Subset:  -    
Affiliation:
Michigan State University-Atomic Energy Commission Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.
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