Document Detail

Phosphorylation of CREB, a cyclic AMP responsive element binding protein, contributes partially to lysophosphatidic acid-induced fibroblast cell proliferation.
MedLine Citation:
PMID:  19285017     Owner:  NLM     Status:  MEDLINE    
Lysophospholipids regulate a wide array of biological processes including cell survival and proliferation. In our previous studies, we found that in addition to SRE, CRE is required for maximal c-fos promoter activation triggered by lysophosphatidic acid (LPA). c-fos is an early indicator of various cells into the cell cycle after mitogenic stimulation. However, role of CREB activation in LPA-stimulated proliferation has not been elucidated yet. Here, we investigate how LPA induces proliferation in Rat-2 fibroblast cell via CREB activation. We found that total cell number and BrdU-positive cells were increased by LPA. Moreover, levels of c-fos mRNA and cyclin D1 protein were increased via LPA-induced CREB phosphorylation. Furthermore, LPA-induced Rat-2 cell proliferation was decreased markedly by ERK inhibitor (U0126) and partially by MSK inhibitor (H89). Taken together, these results suggest that CREB activation could partially up-regulate accumulation of cyclin D1 protein level and proliferation of LPA-stimulated Rat-2 fibroblast cells.
Yong-Jun Kwon; Yuanjie Sun; Nam-Ho Kim; Sung-Oh Huh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-29
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  380     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-04-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  655-9     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University, Chunchon, Gangwon-do 200-702, South Korea.
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MeSH Terms
Cell Proliferation*
Cells, Cultured
Cyclic AMP Response Element-Binding Protein / metabolism*
Cyclin D1 / metabolism*
Fibroblasts / cytology,  drug effects,  metabolism
Lysophospholipids / metabolism*,  pharmacology
Mitogen-Activated Protein Kinase Kinases / metabolism
Proto-Oncogene Proteins c-fos / metabolism
Reg. No./Substance:
0/CREB1 protein, rat; 0/Ccnd1 protein, rat; 0/Cyclic AMP Response Element-Binding Protein; 0/Lysophospholipids; 0/Proto-Oncogene Proteins c-fos; 136601-57-5/Cyclin D1; 22002-87-5/lysophosphatidic acid; EC Protein Kinase Kinases

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