| Phosphoric acid as a matrix additive for MALDI MS analysis of phosphopeptides and phosphoproteins. | |
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MedLine Citation:
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PMID: 15373450 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphopeptides are often detected with low efficiency by MALDI MS analysis of peptide mixtures. In an effort to improve the phosphopeptide ion response in MALDI MS, we investigated the effects of adding low concentrations of organic and inorganic acids during peptide sample preparation in 2,5-dihydroxybenzoic acid (2,5-DHB) matrix. Phosphoric acid in combination with 2,5-DHB matrix significantly enhanced phosphopeptide ion signals in MALDI mass spectra of crude peptide mixtures derived from the phosphorylated proteins alpha-casein and beta-casein. The beneficial effects of adding up to 1% phosphoric acid to 2,5-DHB were also observed in LC-MALDI-MS analysis of tryptic phosphopeptides of B. subtilis PrkC phosphoprotein. Finally, the mass resolution of MALDI mass spectra of intact proteins was significantly improved by using phosphoric acid in 2,5-DHB matrix. |
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Authors:
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Sven Kjellström; Ole Nørregaard Jensen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 76 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-09-17 Completed Date: 2006-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5109-17 Citation Subset: IM |
Copyright Information:
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Copyright 2004 American Chemical Society |
Affiliation:
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Protein Research Group, Department of Biochemistry & Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Bacterial Proteins / chemistry Caseins / chemistry Gentisates / chemistry Humans Ions / chemistry Molecular Sequence Data Peptide Fragments / analysis Peptide Mapping / methods* Phosphopeptides / analysis* Phosphoproteins / chemistry* Phosphoric Acids / chemistry* Phosphorylation Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Trypsin / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Caseins; 0/Gentisates; 0/Ions; 0/Peptide Fragments; 0/Phosphopeptides; 0/Phosphoproteins; 0/Phosphoric Acids; 490-79-9/2,5-dihydroxybenzoic acid; 7664-38-2/phosphoric acid; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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