Document Detail


Phosphonocationic lipids in protein delivery to mice lungs.
MedLine Citation:
PMID:  10756329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cationic liposomes constitute one of the main approaches currently investigated to introduce a gene with therapeutic properties into a cell. Another alternative consists in directly introducing the normal protein of concern to, for example, restore the deleted function. We report here on in vitro and in vivo results obtained with GLB73, one of the phosphonolipids investigated as gene transfer agents. In previous studies this cationic lipid had shown its DNA-transfer efficacy in vitro and in vivo. We also confirmed the feasibility of protein/cationic lipid delivery in epithelial cells of mice lungs after intratracheal administration by use of a reporter gene (beta-galactosidase). Two quantitative tests (i.e., a chemiluminescent assay and a flow cytometry assay) were used to determine the amount of beta-galactosidase found in the lungs and the percentage of transfected cells. They showed that 50% of the cells of mice lungs were still positive at day 4 after protein/GLB73 delivery. Immunohistochemistry and electron microscopy studies allowed us to determine the spatial distribution and visualize the penetration of our complex into the lungs.
Authors:
C Guillaume; P Delépine; B Mercier; E Gobin; J P Leroy; V Morin; C Férec
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical sciences     Volume:  89     ISSN:  0022-3549     ISO Abbreviation:  J Pharm Sci     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-28     Completed Date:  2000-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985195R     Medline TA:  J Pharm Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  639-45     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Affiliation:
Centre de Biogénétique, University Teaching Hospital, ETSBO, BP 454, 29275 Brest Cedex, France. guillaume.christine@wanadoo.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Cations / administration & dosage
Cells, Cultured
Drug Carriers
Drug Delivery Systems
Feasibility Studies
Humans
K562 Cells
Lung / drug effects,  metabolism*
Male
Mice
Phospholipids / administration & dosage*,  chemistry
Proteins / administration & dosage*
Transfection
Chemical
Reg. No./Substance:
0/Cations; 0/Drug Carriers; 0/Phospholipids; 0/Proteins; 0/phosphonolipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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