Document Detail

Phospholipids are synthesized in the G2/M phase of the cell cycle.
MedLine Citation:
PMID:  17113814     Owner:  NLM     Status:  MEDLINE    
Very little is known about the metabolism of phospholipids in the G2 and M phases of the cell cycle, but limited studies have led to the postulation that phospholipid synthesis ceases during this period. To investigate whether phospholipids are synthesized in the G2/M phase of the cell cycle, protocols were developed to produce synchronized MCF-7 cell populations with greater than 80% of the cells in G1/S or G2/M phases that moved in synchrony following removal of the blocking agent. Analysis of the activities of key phosphatidylcholine and phosphatidylethanolamine biosynthetic enzymes in subcellular fractions obtained from MCF-7 cells at different cell cycle phases revealed that there was robust activity of key enzymes in the fractions prepared from MCF-7 cells in G2/M phase. Radiolabeled choline and ethanolamine were rapidly incorporated into cells maintained at G2/M phase with nocodazole, and the rates of incorporation were similar to those obtained in cells allowed to progress into the G1 phase. Furthermore, radiolabeled glycerol was incorporated into phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid in MCF-7 cells maintained at G2/M phase with nocodazole. Similar results were obtained in CHO cells. These results demonstrate that glycerophospholipid synthesis is very active in the G2/M phase of these cells. Therefore, the postulated cessation of phospholipid synthesis in G2/M phases is not applicable to all cell types.
Weiyang Lin; Gilbert Arthur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-21
Journal Detail:
Title:  The international journal of biochemistry & cell biology     Volume:  39     ISSN:  1357-2725     ISO Abbreviation:  Int. J. Biochem. Cell Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-01-19     Completed Date:  2007-04-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508482     Medline TA:  Int J Biochem Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  597-605     Citation Subset:  IM    
Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3.
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MeSH Terms
CHO Cells
Cell Cycle / physiology*
Cell Division / physiology
Cell Line
Choline-Phosphate Cytidylyltransferase / metabolism
G2 Phase / physiology
Phosphatidylcholines / biosynthesis
Phosphatidylethanolamines / biosynthesis
Phospholipids / biosynthesis*
RNA Nucleotidyltransferases / metabolism
S Phase / physiology
Reg. No./Substance:
0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; EC 2.7.7.-/RNA Nucleotidyltransferases; EC cytidylyltransferase; EC Cytidylyltransferase

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