| Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments. | |
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MedLine Citation:
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PMID: 18212025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipids are well known messengers involved in developmental and stress responses mediating intracellular signalling. It has been hypothesized that phospholipids exist which could participate in intercellular communication events through the apoplast of sunflower (Helianthus annuus) seeds. Here it is shown that extracellular washing fluids (EWFs) obtained from seeds imbibed for 2 h contain diverse phospholipids. Lipid profiling by electrospray ionization tandem mass spectrometry revealed that the EWFs have a particular composition, with phosphatidic acid (PA) and phosphatidylinositol (PI) being the major phospholipids. These profiles are clearly distinct from those of seed extract (SE), and comparative SDS-PAGE of EWF and SE, followed by intracellular and plasma membrane marker analyses, allowed a significant contamination of the EWF to be discarded. Treatment of the seeds with 100 microM jasmonic acid (JA) induces changes in the profile of EWF phospholipids, leading to a decrease in PI content, while the accumulation of phosphatidylinositol 4-phosphate (PI4P) and specific PA species is observed. On the other hand, the EWF from seeds subjected to 50 microM abscisic acid (ABA) treatment exhibit an increase in PA and phosphatidylglycerol levels. To our knowledge, this is the first report on the existence of phospholipids as extracellular components of seeds. Moreover, the modulation of PA, PI, and PI4P levels by hormonal treatments further suggests their contribution to intercellular communication in planta. |
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Authors:
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Mariana Regente; Georgina Corti Monzón; Laura de la Canal |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-01-22 |
Journal Detail:
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Title: Journal of experimental botany Volume: 59 ISSN: 1460-2431 ISO Abbreviation: J. Exp. Bot. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-03-17 Completed Date: 2008-05-22 Revised Date: 2009-10-25 |
Medline Journal Info:
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Nlm Unique ID: 9882906 Medline TA: J Exp Bot Country: England |
Other Details:
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Languages: eng Pagination: 553-62 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Buenos Aires, Argentina. mregente@mdp.edu.ar |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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metabolism Cyclopentanes / metabolism Extracellular Fluid / metabolism* Helianthus / metabolism* Oxylipins / metabolism Phospholipids / metabolism* Plant Growth Regulators / metabolism* Seeds / metabolism* Water / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR016475/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cyclopentanes; 0/Oxylipins; 0/Phospholipids; 0/Plant Growth Regulators; 21293-29-8/Abscisic Acid; 6894-38-8/jasmonic acid; 7732-18-5/Water |
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