Document Detail


Phospholipids of Clostridium perfringens: a reexamination.
MedLine Citation:
PMID:  15043870     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The principal fatty acids of phosphatidylethanolamine are myristic acid (14:0), lauric acid (12:0), and palmitic acid (16:0) and the major molecular species are 14:0,14:0 (26.3%); 12:0,14:0 (19.0%); 14:0,16:0 (22.4%) and 16:0,16:0 (17.6%). A similar distribution of molecular species was found in the other major phospholipid, O-alanyl phosphatidylglycerol.
Authors:
Norah C Johnston; John K Baker; Howard Goldfine
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  233     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-26     Completed Date:  2004-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  65-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Chromatography, Thin Layer
Clostridium perfringens / chemistry*
Fatty Acids / chemistry,  isolation & purification
Lauric Acids / chemistry,  isolation & purification
Mass Spectrometry
Myristic Acid / chemistry,  isolation & purification
Palmitic Acid / chemistry,  isolation & purification
Phosphatidylethanolamines / analysis,  chemistry,  isolation & purification
Phosphatidylglycerols / analysis,  chemistry,  isolation & purification
Phospholipids / analysis*,  chemistry*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Lauric Acids; 0/Phosphatidylethanolamines; 0/Phosphatidylglycerols; 0/Phospholipids; 143-07-7/lauric acid; 39382-08-6/phosphatidylethanolamine; 544-63-8/Myristic Acid; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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