| Phospholipid transverse mobility modifications in plasma membranes of activated platelets: an ESR study. | |
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MedLine Citation:
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PMID: 1333195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spin labeled phospholipid analogs were used to directly study changes in aminophospholipid translocase activity in activated platelets. In thrombin-activated platelets, the translocase activity was slightly stimulated, whereas no vesicle formation or proteolysis of cytoskeletal protein occurred. Ca2+ ionophore A23187-mediated activation produced vesiculation and proteolysis. Additionally, the translocase activity was completely inhibited, probably due to a sharp rise the intracellular Ca2+ concentration, as shown when platelets were activated in the presence of various A23187 and Ca2+ concentrations and by the recovery of the translocase activity when Ca2+ was complexed with EGTA. No translocase activity was found in vesicles. Whereas vesiculation and translocase inhibition can occur independently of proteolysis, this later accentuated the shedding phenomenon. |
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Authors:
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F Bassé; P Gaffet; F Rendu; A Bienvenüe |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 189 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1992-12-30 Completed Date: 1992-12-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 465-71 Citation Subset: IM |
Affiliation:
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URA 530 CNRS, Dept Biologie Santé, USTL, Montpellier, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Platelets
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drug effects,
metabolism* Calcimycin / pharmacology Calcium / pharmacology Cell Membrane / drug effects, metabolism* Egtazic Acid / pharmacology Electron Spin Resonance Spectroscopy Humans Kinetics Phospholipids / blood* Platelet Activation* / drug effects Spin Labels Thrombin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 0/Spin Labels; 52665-69-7/Calcimycin; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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