| Phospholipid outside-inside translocation in lymphocyte plasma membranes is a protein-mediated phenomenon. | |
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MedLine Citation:
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PMID: 3801478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have measured the transbilayer diffusion of spin-labeled analogs of sphingomyelin, phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in pig lymphocyte plasma membrane. At 4 degrees C and 37 degrees C the aminophospholipids are rapidly transported from the outer to the inner leaflet of the membrane, whereas the choline-containing phospholipids experience a slower diffusion. This selectivity is abolished after cell treatment by SH-group reagents indicating that the aminophospholipid translocation is protein-dependent and must be driven by a system analogous to the one existing in the human red cell membrane. The fact that the selectivity exists at low temperature, that it does not depend on cytoskeleton integrity and that there is a competition between the two aminophospholipids show that this translocation is not purely an endocytic process. |
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Authors:
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A Zachowski; A Herrmann; A Paraf; P F Devaux |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 897 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 Feb |
Date Detail:
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Created Date: 1987-03-26 Completed Date: 1987-03-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 197-200 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active Cell Membrane / metabolism* Colchicine / pharmacology Cytochalasin B / pharmacology Diffusion Lymphocytes / metabolism* Membrane Fluidity Membrane Lipids / metabolism* Membrane Proteins / physiology* Phospholipids / metabolism* Spin Labels / metabolism Swine Temperature |
| Chemical | |
Reg. No./Substance:
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0/Membrane Lipids; 0/Membrane Proteins; 0/Phospholipids; 0/Spin Labels; 14930-96-2/Cytochalasin B; 64-86-8/Colchicine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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