Document Detail

Phospholipid outside-inside translocation in lymphocyte plasma membranes is a protein-mediated phenomenon.
MedLine Citation:
PMID:  3801478     Owner:  NLM     Status:  MEDLINE    
We have measured the transbilayer diffusion of spin-labeled analogs of sphingomyelin, phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in pig lymphocyte plasma membrane. At 4 degrees C and 37 degrees C the aminophospholipids are rapidly transported from the outer to the inner leaflet of the membrane, whereas the choline-containing phospholipids experience a slower diffusion. This selectivity is abolished after cell treatment by SH-group reagents indicating that the aminophospholipid translocation is protein-dependent and must be driven by a system analogous to the one existing in the human red cell membrane. The fact that the selectivity exists at low temperature, that it does not depend on cytoskeleton integrity and that there is a competition between the two aminophospholipids show that this translocation is not purely an endocytic process.
A Zachowski; A Herrmann; A Paraf; P F Devaux
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  897     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-03-26     Completed Date:  1987-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  197-200     Citation Subset:  IM    
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MeSH Terms
Biological Transport, Active
Cell Membrane / metabolism*
Colchicine / pharmacology
Cytochalasin B / pharmacology
Lymphocytes / metabolism*
Membrane Fluidity
Membrane Lipids / metabolism*
Membrane Proteins / physiology*
Phospholipids / metabolism*
Spin Labels / metabolism
Reg. No./Substance:
0/Membrane Lipids; 0/Membrane Proteins; 0/Phospholipids; 0/Spin Labels; 14930-96-2/Cytochalasin B; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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