| Phospholipid-nucleic acid recognition: developing an immobilized liposome chromatography for DNA separation and analysis. | |
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MedLine Citation:
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PMID: 17089691 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The basic physicochemical principles that determine nucleic acid-phospholipid recognition and self-assembly are presented, focusing on their use as promising stationary phases for separation and analyses of nucleic acids with various structures and properties. The utilization of immobilized liposome chromatography was designed as an aqueous, two-phase system for further studies of sequence- and topology-specific and non-specific nucleic acid binding features of phospholipids in the presence of cationic co-solutes. Such covalently attached liposomes are evaluated for their stability, binding affinities with nucleic acids and plasmids, as well as their subsequent compaction, in terms of their relevance to chromatographic applications. |
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Authors:
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Erhan Süleymanoglu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: PDA journal of pharmaceutical science and technology / PDA Volume: 60 ISSN: 1079-7440 ISO Abbreviation: PDA J Pharm Sci Technol Publication Date: 2006 Jul-Aug |
Date Detail:
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Created Date: 2006-11-08 Completed Date: 2006-12-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9439538 Medline TA: PDA J Pharm Sci Technol Country: United States |
Other Details:
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Languages: eng Pagination: 232-9 Citation Subset: IM |
Affiliation:
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Faculty of Pharmacy, The Central Laboratory and Department of Pharmaceutical Chemistry, Gazi University, Hipodrom, 06330-Ankara, Turkey. esuleymanoglu@gazi.edu.tr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity
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methods* Chromatography, Gel / methods* DNA / analysis*, chemistry* Humans Liposomes Nucleic Acid Conformation* Phospholipids / chemistry* Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 0/Phospholipids; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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