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Phospholipid and nucleic acid gradients in the developing amphibian embryo.
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MedLine Citation:
PMID:  5840800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rana pipiens embryos at the end of the blastula stage were dissociated and the cell suspension was separated into presumptive ectoderm, mesoderm, light endoderm, and heavy endoderm cells by a discontinuous density gradient centrifugation technique. The isolated germ layers were analyzed for total lipid, lipid phosphorus, plasmalogen, RNA, and DNA. Per gram dry weight, DNA showed a threefold decrease from ectoderm to heavy endoderm. On the same basis, the RNA content of the mesoderm was 34 per cent higher than that of ectoderm, and 320 and 570 per cent higher than that of light and heavy endoderm, respectively. In addition to the RNA and DNA gradients, there were at least two superimposed lipid gradients: a neutral lipid gradient decreasing from ectoderm to endoderm, and a total phospholipid gradient increasing from ectoderm to endoderm. In contrast to total phospholipid, a specific phospholipid class, ethanolamine plasmalogen, decreased from ectoderm to endoderm. The total lipid content per gram dry weight was the same in all the germ layers. Total phospholipids were analyzed quantitatively by thin layer chromatography. Phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and inositol phospholipid constituted 34, 13, 12, and 34 per cent, respectively, of the total lipid phosphorus. The phospholipid composition was different in each germ layer. The possible role of specific lipids in embryonic induction and differentiation is discussed.
Authors:
G A Morrill; A B Kostellow
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  25     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1965 Jun 
Date Detail:
Created Date:  1966-01-19     Completed Date:  1966-01-19     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  Suppl:21-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Anura
Centrifugation
Chemical Phenomena
Chemistry
Chemistry Techniques, Analytical
DNA*
Ectoderm*
Embryo, Nonmammalian*
Endoderm*
Mesoderm*
Phospholipids*
RNA*
Chemical
Reg. No./Substance:
0/Phospholipids; 63231-63-0/RNA; 9007-49-2/DNA
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1965 by The Rockefeller Institute Press
Received Day: 8 Month: 7 Year: 1964
Print publication date: Day: 1 Month: 6 Year: 1965
Volume: 25 Issue: 3
First Page: 21 Last Page: 29
ID: 2106670
PubMed Id: 5840800

PHOSPHOLIPID AND NUCLEIC ACID GRADIENTS IN THE DEVELOPING AMPHIBIAN EMBRYO
Gene A. Morrill
Adele B. Kostellow
From the Department of Physiology, Albert Einstein College of Medicine of Yeshiva University, New York


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