Document Detail


Phospholipid metabolism in V79-R membranes composed of phospholipid molecular species containing trans-monoenoic fatty acids.
MedLine Citation:
PMID:  6094524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interrelationship between the inhibition of cell growth and changes in phospholipid molecular species was studied in the presence of elaidic, trans-11-eicosenoic, or brassidic acids in Chinese hamster V79-R cells. The addition of trans-monoenoic fatty acids to the medium inhibited cell growth and caused an increase in the total cellular content of phospholipids. However, there was no difference in the polar head group composition of these phospholipids among all the cells supplemented with trans-monoenoic fatty acids. Exogenous trans-monoenoic fatty acids were incorporated into cellular phospholipids to form novel phospholipid molecular species. Phospholipid synthesizing enzyme activities bound to the membranes composed of phospholipid molecular species of trans-monoenoic fatty acids were determined. Cholinephosphotransferase [EC 2.7.8.2] and ethanolaminephosphotransferase [EC 2.7.8.1] activities were decreased by trans-11-eicosenoic acid, but not changed by elaidic acid. Glycerophosphate acyltransferase [EC 2.3.1.15] activity was increased by elaidic acid and decreased by trans-11-eicosenoic acid. Cholinephosphate cytidylyltransferase [EC 2.7.7.15] activity was not changed by trans-monoenoic fatty acids.
Authors:
R Urade; M Kito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  96     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1985-01-14     Completed Date:  1985-01-14     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  821-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Cell Line
Cell Membrane / metabolism
Choline-Phosphate Cytidylyltransferase
Cricetinae
Cricetulus
Diacylglycerol Cholinephosphotransferase / metabolism
Ethanolaminephosphotransferase / metabolism
Fatty Acids, Unsaturated / isolation & purification,  metabolism*,  pharmacology
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Kinetics
Lung
Membrane Lipids / metabolism*
Nucleotidyltransferases / metabolism
Phospholipids / isolation & purification,  metabolism*
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Fatty Acids, Unsaturated; 0/Membrane Lipids; 0/Phospholipids; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.15/Choline-Phosphate Cytidylyltransferase; EC 2.7.8.1/Ethanolaminephosphotransferase; EC 2.7.8.2/Diacylglycerol Cholinephosphotransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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