| Phospholipid metabolism during bacterial growth. | |
| | |
MedLine Citation:
|
PMID: 4305713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Haemophilus parainfluenzae incorporates glycerol and phosphate into the membrane phospholipids without lag during logarithmic growth. In phosphatidyl glycerol (PG), the phosphate and unacylated glycerol moieties turn over and incorporate radioactivity much more rapidly than does the diacylated glycerol. At least half the radioactivity is lost from the phosphate and unacylated glycerol in about 1 doubling. The total fatty acids turn over slightly faster than the diacyl glycerol. In phosphatidyl ethanolamine (PE), which is the major lipid of the bacterium, ethanolamine and phosphate turn over and incorporate radioactivity at least half as fast as the phosphate in PG. The glycerol of PE did not turn over in 4 bacterial doublings. In phosphatidic acid the glycerol turns over at one-third the rate of phosphate turnover. By means of a modified method for the quantitative recovery of 1,3-glycerol diphosphate from cardiolipin, the phosphates and middle glycerol of cardiolipin were shown to turn over more rapidly than the acylated glycerols during bacterial growth. There is no randomization of the radioactivity in the 1- and 3-positions of the glycerol in the course of 1 doubling. The fatty acids of PG turn over faster than those in PE. In both lipids the 2-fatty acids turn over much faster than the 1-fatty acids. At both positions the individual fatty acids have their own rates of turnover. The distribution of fatty acids between the 1- and 2-positions is the same as in other organisms, with more monoenoic and long-chain fatty acids at the 2-position. The different rates of turnover and incorporation of radioactivity into different parts of the lipids suggest that exchange reactions may be important to phospholipid metabolism. |
| | |
Authors:
|
D C White; A N Tucker |
Related Documents
:
|
6407533 - Formation of diacylglycerol and degradation of phosphatidylinositol induced in rat lymp... 2864143 - Synthesis of 5,9-hexacosadienoic acid phospholipids. 11. phospholipid studies of marine... 22284573 - Hydrothermal synthesis of cuo micro-/nanostructures and their applications in the oxida... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of lipid research Volume: 10 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1969 Mar |
Date Detail:
|
Created Date: 1969-06-24 Completed Date: 1969-06-24 Revised Date: 2003-11-04 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 220-33 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Alcohols
/
metabolism Carbon Isotopes Cell Division Cell Membrane Chromatography, Gas Chromatography, Ion Exchange Chromatography, Thin Layer Fatty Acids / metabolism Glycerol / metabolism Glycerophosphates / metabolism Haemophilus / growth & development, metabolism* Periodic Acid Phosphates / metabolism Phosphatidylethanolamines / metabolism Phospholipases Phospholipids / metabolism* Phosphorus Isotopes Serine / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amino Alcohols; 0/Carbon Isotopes; 0/Fatty Acids; 0/Glycerophosphates; 0/Phosphates; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Phosphorus Isotopes; 10450-60-9/Periodic Acid; 56-45-1/Serine; 56-81-5/Glycerol; EC 3.1.-/Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mechanism of squalene biosynthesis: evidence against the involvement of free nerolidyl pyrophosphate...
Next Document: Phosphorus determination in phosphoglycerides from thin-layer chromatograms.