| Phospholipid hydroperoxide glutathione peroxidase induces a delay in G1 of the cell cycle. | |
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MedLine Citation:
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PMID: 12868489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipid hydroperoxide glutathione peroxidase (PhGPx) is an antioxidant enzyme that reduces cellular phospholipid hydroperoxides (PLOOHs) to alcohols. Cellular peroxide tone has been implicated in cell growth and differentiation. By reducing the PLOOH level in the cell membrane, PhGPx regulates the peroxide tone and thereby might be involved in cell growth. We hypothesized that overexpression of PhGPx in human breast cancer cells would decrease their growth rate. We stably transfected MCF-7 cells (Wt) with L-PhGPx and measured cell doubling time, plating efficiency, and cell cycle phase transit times. P-4 cells (8-fold increase in PhGPx activity) showed a 2-fold increase in doubling time; doubling time increased directly with PhGPx activity (r = 0.95). The higher the PhGPx activity, the lower the plating efficiency (r = -0.86). The profile of other antioxidant enzymes was unchanged. Overexpression of PhGPx lowered the steady-state level of PLOOH (by > 60%). Results from bromodeoxyuridine pulse-chase experiments and flow cytometry indicate that PhGPx induced a delay in MCF-7 proliferation that was primarily due to a slower progression from G1 to S. These results support the hypothesis that PhGPx plays a regulatory role in the progression of MCF-7 cells from G1 to S possibly by regulating the steady-state levels of PLOOH. These data suggest that PhGPx can lower the peroxide tone, which might change the cellular redox environment resulting in a delay in G1 transit. Thus, PhGPx could be an important factor in cell growth. |
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Authors:
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Hong P Wang; Freya Q Schafer; Prabhat C Goswami; Larry W Oberley; Garry R Buettner |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Free radical research Volume: 37 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-18 Completed Date: 2003-12-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 621-30 Citation Subset: IM |
Affiliation:
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Free Radical and Radiation Biology and The Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242-1101, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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metabolism Blotting, Western Bromodeoxyuridine / pharmacology Catalase / metabolism Cell Cycle Cell Differentiation Cell Line, Tumor Flow Cytometry G1 Phase* Glutathione / metabolism Glutathione Peroxidase / metabolism* Glutathione Reductase / metabolism Humans Immunoblotting Lipid Metabolism Lipid Peroxides / metabolism Models, Chemical Oxidation-Reduction Protein Biosynthesis Superoxide Dismutase / metabolism Time Factors Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA66081/CA/NCI NIH HHS; CA69593/CA/NCI NIH HHS; CA81090/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Lipid Peroxides; 59-14-3/Bromodeoxyuridine; 70-18-8/Glutathione; EC 1.11.1.12/phospholipid-hydroperoxide glutathione peroxidase; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase; EC 1.8.1.7/Glutathione Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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