| Phospholipid furan fatty acids and ubiquinone-8: lipid biomarkers that may protect dehalococcoides strains from free radicals. | |
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MedLine Citation:
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PMID: 16332831 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dehalococcoides species have a highly restricted lifestyle and are only known to derive energy from reductive dehalogenation reactions. The lipid fraction of two Dehalococcoides isolates, strains BAV1 and FL2, and a tetrachloroethene-to-ethene-dechlorinating Dehalococcoides-containing consortium were analyzed for neutral lipids and phospholipid fatty acids. Unusual phospholipid modifications, including the replacement of unsaturated fatty acids with furan fatty acids, were detected in both Dehalococcoides isolates and the mixed culture. The following three furan fatty acids are reported as present in bacterial phospholipids for the first time: 9-(5-pentyl-2-furyl)-nonanoate (Fu18:2omega6), 9-(5-butyl-2-furyl)-nonanoate (Fu17:2omega5), and 8-(5-pentyl-2-furyl)-octanoate (Fu17:2omega6). The neutral lipids of the Dehalococcoides cultures contained unusually large amounts of benzoquinones (i.e., ubiquinones [UQ]), which is unusual for anaerobes. In particular, the UQ-8 content of Dehalococcoides was 5- to 20-fold greater than that generated in aerobically grown Escherichia coli cultures relative to the phospholipid fatty acid content. Naphthoquinone isoprenologues (MK), which are often found in anaerobically grown bacteria and archaea, were also detected. Dehalococcoides shows a difference in isoprenologue pattern between UQ-8 and MK-5 that is atypical of other bacteria capable of producing both quinone types. The difference in UQ-8 and MK-5 isoprenologue patterns strongly suggests a special function for UQ in Dehalococcoides, and Dehalococcoides may utilize structural modifications in its lipid armamentarium to protect against free radicals that are generated in the process of reductive dechlorination. |
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Authors:
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David C White; Roland Geyer; Aaron D Peacock; David B Hedrick; Stephen S Koenigsberg; Youlboong Sung; Jianzhong He; Frank E Löffler |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 71 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-07 Completed Date: 2006-02-07 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 8426-33 Citation Subset: IM |
Affiliation:
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Center for Biomarker Analysis, The University of Tennessee, Knoxville, TN 37932, USA. dwhite1@utk.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biofilms
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classification Biomass Chloroflexi / drug effects, growth & development, isolation & purification, physiology* Culture Media Fatty Acids / classification, metabolism* Free Radicals / toxicity* Linoleic Acids / metabolism* Mass Spectrometry Phospholipids / metabolism* Quinones / metabolism Ubiquinone / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Free Radicals; 0/Linoleic Acids; 0/Phospholipids; 0/Quinones; 1339-63-5/Ubiquinone; 2394-68-5/ubiquinone 8 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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