Document Detail


Phospholipid and fatty acid compositions of Rhizobium leguminosarum biovar trifolii ANU843 in relation to flavone-activated pSym nod gene expression.
MedLine Citation:
PMID:  8277828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipid and associated fatty acid compositions of the bacterial symbiont of clover, Rhizobium leguminosarum biovar trifolii wild-type ANU843, was analyzed by two-dimensional silica thin-layer chromatography, fast atom bombardment-mass spectrometry, flame-ionization detection gas-liquid chromatography and combined gas-liquid chromatography/mass spectrometry. The phospholipid composition included phosphatidylethanolamine (15%), N-methylphosphatidylethanolamine (47%), N,N-dimethylphosphatidylethanolamine (9%), phosphatidylglycerol (19%), cardiolipin (5%) and phosphatidylcholine (2%). Fatty acid composition included predominantly cis-11-octadecenoic acid, lower levels of cis-9-hexadecenoic acid, hexadecanoic acid, 11-methyl-11-octadecenoic acid, octadecanoic acid, 11,12-methyleneoctadecanoic acid, eicosanoic acid and traces of branched, and di- and triunsaturated fatty acids. The influence of expression of the "nodulation" genes encoding symbiotic functions on the composition of these membrane lipids was examined in wild-type cells grown with or without the flavone inducer, 4',7-dihydroxyflavone and in mutated cells lacking the entire symbiotic plasmid where these genes reside, or containing single transposon insertions in selected nodulation genes. No significant changes in phospholipid or associated fatty acid compositions were detected by the above methods of analysis.
Authors:
G G Orgambide; Z H Huang; D A Gage; F B Dazzo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  28     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-02-09     Completed Date:  1994-02-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  975-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Michigan State University, East Lansing 48824.
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MeSH Terms
Descriptor/Qualifier:
Fatty Acids / analysis*
Flavonoids / pharmacology*
Gene Expression Regulation, Bacterial / drug effects*
Genes, Bacterial
Nitrogen Fixation
Phospholipids / analysis*
Plasmids / genetics
Rhizobium leguminosarum / chemistry*,  genetics
Spectrometry, Mass, Fast Atom Bombardment
Symbiosis / genetics
Grant Support
ID/Acronym/Agency:
RR00480/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Flavonoids; 0/Phospholipids; 2196-14-7/4',7-dihydroxyflavone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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