Document Detail


Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.
MedLine Citation:
PMID:  12127500     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phospholipid etherlipid (PLEL) derived isoprenoids and phospholipid fatty acids (PLFA) were determined in eight Euryarchaeotal monocultures for taxonomic profiling. For the first time significant amounts of fatty acids in the PLFA of Euryarchaeota were determined. The PLFA proportion varied between 11.3 and 35.5% of the total phospholipid side chains except in Methanothermus fervidus where PLFA accounted for 89.0% of the total phospholipid side chains. Fractionation of fatty acids prior to gas chromatography mass spectrometry analysis revealed that non-ester-linked fatty acids dominated which accounted for 85.5-95.2% of total PLFA in all investigated archaeal strains. PLEL concentration and composition was estimated in accordance with previous studies with two exceptions. In the polar (phospho)lipid fraction of Methanopyrus kandleri side chains possibly derived from hydroxyarchaeol as well as acyclic and cyclic caldarchaeol were identified. In phospholipid extracts of Methanothermus fervidus the 'H-formed' caldarchaeol could not be detected. Overall, PLEL derived isoprenoids as well as PLFA enabled taxonomic differentiation of the selected microorganisms into phylogenetically related groups.
Authors:
Andreas Gattinger; Michael Schloter; Jean Charles Munch
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  213     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-19     Completed Date:  2003-04-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  133-9     Citation Subset:  IM    
Affiliation:
Institute of Soil Ecology, GSF-National Research Center for Environment and Health, Ingolstädter Landstr. 1, Neuherberg, Germany. gattinger@gsf.de
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MeSH Terms
Descriptor/Qualifier:
Classification / methods
Euryarchaeota / chemistry,  classification*
Fatty Acids / analysis*
Phospholipids / analysis*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phospholipids

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