| Phospholipid degradation in energy-deprived cardiac myocytes: does annexin V play a role? | |
| | |
MedLine Citation:
|
PMID: 9201625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study explored whether annexin V, a protein with established phospholipase A2 inhibiting properties, plays a role in the degradation of membrane phospholipids of adult cardiac myocytes during metabolic inhibition (20 mM 2-deoxyglucose and 1 mM iodoacetic acid). Experiments were carried out on isolated cardiac myocytes prelabeled with [14C]-arachidonic acid, which were subjected to metabolic inhibition for up to 240 min. Under control conditions, annexin V was found to be localised predominantly at the sarcolemma. After 120 min of metabolic inhibition, the release of lactate dehydrogenase (LDH) was still comparable with control cells, while morphological changes were already visible. After 240 min of metabolic inhibition, LDH release was significantly elevated compared to control cells incubated for the same period of time (35% v 20% of total cellular activity). All myocytes had lost their typical elongated shape and sarcolemmal "blebs" had been formed. In metabolically inhibited cells, annexin V localisation seemed to be more pronounced at the level of the sarcolemma compared to controls, whereas membrane phospholipid hydrolysis occurred at a significantly elevated rate, as evidenced by a significantly enhanced accumulation of labeled arachidonic acid within the cells. The present findings are not in favor of the hypothesis that the increase in net degradation of phospholipids in energy-deprived cardiac myocytes is caused by a loss of annexin V from the sarcolemma, which would increase the vulnerability of the sarcolemma to phospholipase A2 activity. |
| | |
Authors:
|
S W Jans; J Willems; M van Bilsen; C P Reutelingsperger; G J van der Vusse |
Related Documents
:
|
7863525 - Chronic inhalation exposure and phospholipids in lung surfactant and tissue. 2807145 - The "survival hormones": azelaic and pimelic acids, suppress the stress elicited by iso... 2382705 - Selective turnover of sarcolemmal phospholipids with lethal cardiac myocyte injury. 15256315 - Fatty acid composition of erythrocyte phospholipids is related to insulin levels, secre... 168535 - The origin of proteins: heteropolypeptides from hydrogen cyanide and water. 22055945 - Effect of acid decontamination of beef subprimal cuts on the microbiological and sensor... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of molecular and cellular cardiology Volume: 29 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 1997 May |
Date Detail:
|
Created Date: 1997-09-24 Completed Date: 1997-09-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1401-10 Citation Subset: IM |
Affiliation:
|
Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Annexin A5 / metabolism* Arachidonic Acid / metabolism Carbon Radioisotopes Cells, Cultured Energy Metabolism Fatty Acids / metabolism L-Lactate Dehydrogenase / secretion Male Myocardium / cytology, metabolism* Phospholipids / metabolism* Rats Rats, Inbred Lew Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A5; 0/Carbon Radioisotopes; 0/Fatty Acids; 0/Phospholipids; 0/Triglycerides; 506-32-1/Arachidonic Acid; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Beta-adrenergic receptor signalling in stunned myocardium of conscious pigs.
Next Document: Cyclic nucleotides regulate the activity of L-type calcium channels in smooth muscle cells from rat ...