Document Detail


Phospholipid-cytochrome c interaction: evidence for the extended lipid anchorage.
MedLine Citation:
PMID:  11781329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Binding of cytochrome c (cyt c) to fatty acids and acidic phospholipid membranes produces pronounced and essentially identical changes in the spectral properties of cyt c, revealing conformational changes in the protein. The exact mechanism of the interaction of fatty acids and acidic phospholipids with cyt c is unknown. Binding of cyt c to liposomes with high contents (mole fraction X > 0.7) of acidic phospholipids caused spectral changes identical to those due to binding of oleic acid. Fluorescence spectroscopy of a cyt c analog containing a Zn(2+) substituted heme moiety and brominated lipid derivatives (9,10)-dibromostearate and 1-palmitoyl-2-(9,10)-dibromo-sn-glycero-3-phospho-rac-glycerol demonstrated a direct contact between the fluorescent [Zn(2+)-heme] group and the brominated acyl chain. These data constitute direct evidence for interaction between an acyl chain of a membrane phospholipid and the inside of the protein containing the heme moiety and provide direct evidence for the so-called extended-lipid anchorage of cyt c to phospholipid membranes. In this mechanism, one of the phospholipid acyl chains protrudes out of the membrane and intercalates into a hydrophobic channel in cyt c while the other chain remains in the bilayer.
Authors:
Esa K J Tuominen; Carmichael J A Wallace; Paavo K J Kinnunen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-01-07
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  277     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-11     Completed Date:  2002-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8822-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Helsinki Biophysics and Biomembrane Group, Institute of Biomedicine, P. O. Box 63, Haartmaninkatu 8, University of Helsinki, FIN-00014 Finland.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Cytochrome c Group / chemistry,  metabolism*
Membrane Lipids / metabolism*
Phospholipids / metabolism*
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Cytochrome c Group; 0/Membrane Lipids; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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