| Phospholipid compositional changes of five pseudomonad archetypes grown with and without toluene. | |
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MedLine Citation:
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PMID: 11030576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial physiological responses to toluene exposure were investigated in five reference pseudomonad strains that express different toluene degradation pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1. The intact phospholipids of these archetypes, grown with and without toluene, were characterized using liquid chromatography/electrospray ionization/mass spectrometry. All strains showed significant changes in phospholipid content and composition as an adaptive response to toluene exposure, as well as considerable diversity in response mechanisms. For example, the phospholipid content of toluene-grown PKO1, F1, and KR1 were 10.9-34.7% of that found in succinate-grown strains, while the phospholipid content of mt-2 and G4 increased by 56% and 94%, respectively, when grown on toluene. In addition, PKO1, F1, and mt-2 responded to the presence of toluene by synthesizing more phosphatidylglycerol, whereas G4 and KR1 synthesized phospholipids with polyunsaturated fatty acids (C18:2) on one or both of the sn-2 positions. These changes in phospholipid composition and concentration probably reflect the sensitivity and degree of tolerance of these strains to toluene, and suggest that different mechanisms are utilized by dissimilar bacteria to maintain optimal lipid ordering in the presence of such environmental pollutants. |
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Authors:
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J Fang; M J Barcelona; P J Alvarez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 54 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2001-01-09 Completed Date: 2001-01-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 382-9 Citation Subset: IM |
Affiliation:
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Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor 48109-2099, USA. jsfang@engin.umich.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Burkholderia
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chemistry*,
growth & development,
metabolism Burkholderia cepacia / chemistry, growth & development, metabolism Cell Membrane / chemistry Culture Media Phospholipids / analysis* Pseudomonas / chemistry*, growth & development, metabolism Pseudomonas putida / chemistry, growth & development, metabolism Succinates / metabolism Toluene / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Phospholipids; 0/Succinates; 108-88-3/Toluene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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