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Phospholipid composition of several clinically relevant Corynebacterium species as determined by mass spectrometry: an unusual fatty acyl moiety is present in inositol-containing phospholipids of Corynebacterium urealyticum.
MedLine Citation:
PMID:  12855719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparative study on phospholipids of Corynebacterium amycolatum, Corynebacterium jeikeium and Corynebacterium urealyticum was carried out using fast-atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry. Data obtained indicate the presence of acylphosphatidylglycerol (APG), diphosphatidylglycerol, phosphatidylglycerol (PG), phosphatidylinositol (PI) and triacylphosphatidylinositol dimannosides (Ac(3)PIM(2)) in these bacteria. In general, octadecenoyl and hexadecanoyl fatty acyl moieties predominated in phospholipids of C. amycolatum, whereas high levels of hexadecenoyl were found in C. jeikeium and C. urealyticum. Mass spectra from purified APG and PG indicated that the sn-1 position of the glycerol was occupied by octadecenoyl in the three species studied. Notably, several major molecular species of PI and Ac(3)PIM(2) from C. urealyticum contained significant amounts of a moiety identified as 10-methyleneoctadecanoyl, located at the sn-1 position of these molecules. On the other hand, multiantibiotic resistant and susceptible strains of C. amycolatum differed in several minor phospholipid fatty acids of 19 carbon atoms, identified as 10-methyloctadecenoic, 10-methyloctadecanoic (tuberculostearic acid) and 10-methyleneoctadecanoic. The results demonstrate an overall similarity among the phospholipids of the different species studied but also significant differences related to the acyl chains of the glycerol moiety of these compounds, notably the high levels of an unusual fatty acyl moiety in inositol-containing phospholipids of C. urealyticum.
Authors:
Genoveva Yagüe; Manuel Segovia; Pedro L Valero-Guillén
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  149     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-11     Completed Date:  2003-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1675-85     Citation Subset:  IM    
Affiliation:
Departamento de Genética y Microbiología, Facultad de Medicina, Universidad de Murcia, Campus Universitario de Espinardo, 30100 Murcia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Corynebacterium / chemistry*
Fatty Acids / chemistry
Phosphatidylcholines / chemistry
Phosphatidylinositols / chemistry
Phospholipids / chemistry*
Species Specificity
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Fast Atom Bombardment
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylinositols; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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