| Phospholipid composition and metabolism of Micrococcus denitrificans. | |
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MedLine Citation:
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PMID: 4640503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The phospholipid composition of Micrococcus denitrificans was unusual in that phosphatidyl choline (PC) was a major phospholipid (30.9%). Other phospholipids were phosphatidyl glycerol (PG, 52.4%), phosphatidyl ethanolamine (PE, 5.8%), an unknown phospholipid (5.3%), cardiolipin (CL, 3.2%), phosphatidyl dimethylethanolamine (PDME, 0.9%), phosphatidyl monomethylethanolamine (PMME, 0.6%), phosphatidyl serine (PS, 0.5%), and phosphatidic acid (0.4%). Kinetics of (32)P incorporation suggested that PC was formed by the successive methylations of PE. Pulse-chase experiments with pulses of (32)P or acetate-1-(14)C to exponentially growing cells showed loss of isotopes from PMME, PDME, PS, and CL with biphasic kinetics suggesting the same type of multiple pools of these lipids as proposed in other bacteria. The major phospholipids, PC, PG, and PE, were metabolically stable under these conditions. The fatty acids isolated from the complex lipids were also unusual in being a simple mixture of seven fatty acids with oleic acid representing 86% of the total. Few free fatty acids and no non-extractable fatty acids associated with the cell wall or membrane were found. |
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Authors:
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B J Wilkinson; M R Morman; D C White |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 112 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1972 Dec |
Date Detail:
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Created Date: 1973-02-15 Completed Date: 1973-02-15 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1288-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoradiography Carbon Isotopes Cardiolipins / analysis Cell Membrane / analysis Chromatography, Gas Chromatography, Thin Layer Electron Transport Fatty Acids / analysis Fatty Acids, Nonesterified / analysis Glycerol / analysis Lipids / analysis, isolation & purification Micrococcus / analysis* Paracoccus denitrificans / analysis, growth & development, metabolism Phosphatidylcholines / analysis Phosphatidylethanolamines / analysis Phospholipids / analysis*, metabolism Phosphorus Isotopes |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Cardiolipins; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Phosphorus Isotopes; 56-81-5/Glycerol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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