Document Detail


Phospholipid composition of the granular amebocyte from the horseshoe crab, Limulus polyphemus.
MedLine Citation:
PMID:  9778141     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipid composition was determined for the amebocyte of the primitive arthropod Limulus polyphemus. The total fatty acid composition of the cells' lipids was analyzed by gas chromatography/mass spectrometry (GC/MS) of fatty acid methyl esters (FAME). The FAME analysis revealed high levels of 20-carbon polyunsaturated fatty acids (PUFA), especially arachidonic (20:4n-6) and eicosapentaenoic (20:5n-3) acids. Almost 20% of the total lipid profile was comprised of dimethyl acetals of 16- to 20-carbon chain lengths, indicative of plasmalogens in the phospholipid pool. Phospholipids, analyzed by high-pressure liquid chromatography, included phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SPH), and cardiolipin (CL). PE and PC levels predominated at 42.2 and 36.3%, respectively. Smaller amounts of PS (9.0%) and PI (6.2%) were present, as well as low levels of SPH (4.6%), CL (1.6%), and trace amounts of lysophosphatidylcholine. The major phospholipid species, PE, PC, PS and PI, were collected and their molecular species were examined by electrospray-ionization mass spectrometry. The molecular species within the phospholipid classes reflected the high levels of PUFA seen in the total lipid profile. PI was mainly composed of 18:0a/20:4. Over half of the PS consisted of 18:0a/18:1 and 18:0a/20:4. The major PE species were 20:1p/20:5, 20:1p/20:4, 18:0p/20:5, and 18:0p/20:4. PC had the largest distribution of molecular species, and its most abundant species were 16:0e/20:5, 16:0e/20:4, and 16:0p/20:4. The presence of 16:0e/20:4 is the first documentation of a specific precursor to platelet-activating factor in an invertebrate hemocyte. Note: at the sn-1 position: [a=1=O-acyl, e = 1-O-alkylether, and p = 1-O-alk-1'-enyl (plasmalogen)].
Authors:
J C MacPherson; J G Pavlovich; R S Jacobs
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  33     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-30     Completed Date:  1998-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  931-40     Citation Subset:  IM    
Affiliation:
Marine Science Institute, University of California, Santa Barbara 93106, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiolipins / analysis
Chromatography, Gas
Chromatography, High Pressure Liquid
Fatty Acids / analysis*,  metabolism
Horseshoe Crabs / chemistry*
Lysophosphatidylcholines / analysis
Mass Spectrometry / methods
Phospholipids / blood*,  chemistry*,  classification
Sphingomyelins / analysis
Chemical
Reg. No./Substance:
0/Cardiolipins; 0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Phospholipids; 0/Sphingomyelins

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