Document Detail


Phospholipid composition of articular cartilage boundary lubricant.
MedLine Citation:
PMID:  11518278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism of lubrication in normal human joints depends on loading and velocity conditions. Boundary lubrication, a mechanism in which layers of molecules separate opposing surfaces, occurs under severe loading. This study was aimed at characterizing the phospholipid composition of the adsorbed molecular layer on the surface of normal cartilage that performs as a boundary lubricant. The different types of phospholipid adsorbed onto the surface of cartilage were isolated by extraction and identified by chromatography on silica gel paper and mass spectroscopy. The main phospholipid classes identified were quantified by a phosphate assay. Gas chromatography and electrospray ionization mass spectrometry were used to further characterize the fatty acyl chains in each major phospholipid component and to identify the molecular species present. Phosphatidylcholine (41%), phosphatidylethanolamine (27%) and sphingomyelin (32%) were the major components of the lipid layer on the normal cartilage surface. For each lipid type, a mixture of fatty acids was detected, with a higher percentage of unsaturated species compared to saturated species. The most abundant fatty acid observed with all three lipid types was oleic acid (C18:1). Additional work to further quantify the molecular species using electrospray ionization mass spectrometry is recommended.
Authors:
A V Sarma; G L Powell; M LaBerge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  19     ISSN:  0736-0266     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-23     Completed Date:  2001-09-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  671-6     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, Clemson University, SC 29634-0905, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / chemistry*
Cattle
Fatty Acids / analysis
Fatty Acids, Unsaturated / analysis
Phosphatidylcholines / analysis
Phosphatidylethanolamines / analysis
Phospholipids / analysis*
Spectrometry, Mass, Electrospray Ionization
Sphingomyelins / analysis
Grant Support
ID/Acronym/Agency:
RR07141/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Sphingomyelins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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