| Phospholipid composition of articular cartilage boundary lubricant. | |
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MedLine Citation:
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PMID: 11518278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of lubrication in normal human joints depends on loading and velocity conditions. Boundary lubrication, a mechanism in which layers of molecules separate opposing surfaces, occurs under severe loading. This study was aimed at characterizing the phospholipid composition of the adsorbed molecular layer on the surface of normal cartilage that performs as a boundary lubricant. The different types of phospholipid adsorbed onto the surface of cartilage were isolated by extraction and identified by chromatography on silica gel paper and mass spectroscopy. The main phospholipid classes identified were quantified by a phosphate assay. Gas chromatography and electrospray ionization mass spectrometry were used to further characterize the fatty acyl chains in each major phospholipid component and to identify the molecular species present. Phosphatidylcholine (41%), phosphatidylethanolamine (27%) and sphingomyelin (32%) were the major components of the lipid layer on the normal cartilage surface. For each lipid type, a mixture of fatty acids was detected, with a higher percentage of unsaturated species compared to saturated species. The most abundant fatty acid observed with all three lipid types was oleic acid (C18:1). Additional work to further quantify the molecular species using electrospray ionization mass spectrometry is recommended. |
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Authors:
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A V Sarma; G L Powell; M LaBerge |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: 19 ISSN: 0736-0266 ISO Abbreviation: J. Orthop. Res. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-08-23 Completed Date: 2001-09-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: United States |
Other Details:
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Languages: eng Pagination: 671-6 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, Clemson University, SC 29634-0905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage, Articular / chemistry* Cattle Fatty Acids / analysis Fatty Acids, Unsaturated / analysis Phosphatidylcholines / analysis Phosphatidylethanolamines / analysis Phospholipids / analysis* Spectrometry, Mass, Electrospray Ionization Sphingomyelins / analysis |
| Grant Support | |
ID/Acronym/Agency:
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RR07141/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Sphingomyelins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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