| Phospholipid class as a determinant in docosahexaenoic acid's effect on tumor cell viability. | |
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MedLine Citation:
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PMID: 8917399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here we explore how incorporation of the omega-3 fatty acid docosahexaenoic acid (DHA) into murine leukemia cells (T27A) may alter membrane structure and function. When cells were cultured in DHA-supplemented medium, DHA incorporated rapidly and preferentially into phosphatidyl-ethanolamine (PE), with lesser and slower incorporation into phosphatidylcholine (PC). DHA at low concentrations preferred PE over neutral lipids, but in DHA excess accumulation in neutral lipids outstripped that of phospholipids. High DHA levels reduced cell growth in the apparent absence of lipid peroxidation. To study the importance of DHA's phospholipid class, cells were fused with lipid vesicles of either 18:0, 22:6 PE or 18:0, 22:6 PC. DHA-containing PC vesicles produced a dose-dependent decrease in cell viability, whereas PE-containing vesicles had little effect although they appeared more fusogenic. These results provoke the interesting speculation that T27A cells can safely accumulate DHA in PE, but are vulnerable if excessive DHA is incorporated into PC. |
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Authors:
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M Zerouga; W Stillwell; J Stone; A Powner; L J Jenski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Anticancer research Volume: 16 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 1996 Sep-Oct |
Date Detail:
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Created Date: 1996-12-18 Completed Date: 1996-12-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
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Languages: eng Pagination: 2863-8 Citation Subset: IM |
Affiliation:
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Department of Biology, Indiana University, Purdue University at Indianapolis 46202-5132, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cell Survival / drug effects Docosahexaenoic Acids / chemistry, pharmacokinetics*, pharmacology Dose-Response Relationship, Drug Lipid Peroxidation Membrane Lipids / metabolism* Phosphatidic Acids / metabolism* Phosphatidylcholines / metabolism* Phospholipids / metabolism Tumor Cells, Cultured / drug effects, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01CA57212/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Lipids; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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