Document Detail


Phospholipid class as a determinant in docosahexaenoic acid's effect on tumor cell viability.
MedLine Citation:
PMID:  8917399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we explore how incorporation of the omega-3 fatty acid docosahexaenoic acid (DHA) into murine leukemia cells (T27A) may alter membrane structure and function. When cells were cultured in DHA-supplemented medium, DHA incorporated rapidly and preferentially into phosphatidyl-ethanolamine (PE), with lesser and slower incorporation into phosphatidylcholine (PC). DHA at low concentrations preferred PE over neutral lipids, but in DHA excess accumulation in neutral lipids outstripped that of phospholipids. High DHA levels reduced cell growth in the apparent absence of lipid peroxidation. To study the importance of DHA's phospholipid class, cells were fused with lipid vesicles of either 18:0, 22:6 PE or 18:0, 22:6 PC. DHA-containing PC vesicles produced a dose-dependent decrease in cell viability, whereas PE-containing vesicles had little effect although they appeared more fusogenic. These results provoke the interesting speculation that T27A cells can safely accumulate DHA in PE, but are vulnerable if excessive DHA is incorporated into PC.
Authors:
M Zerouga; W Stillwell; J Stone; A Powner; L J Jenski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  16     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1996 Sep-Oct
Date Detail:
Created Date:  1996-12-18     Completed Date:  1996-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2863-8     Citation Subset:  IM    
Affiliation:
Department of Biology, Indiana University, Purdue University at Indianapolis 46202-5132, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Cell Survival / drug effects
Docosahexaenoic Acids / chemistry,  pharmacokinetics*,  pharmacology
Dose-Response Relationship, Drug
Lipid Peroxidation
Membrane Lipids / metabolism*
Phosphatidic Acids / metabolism*
Phosphatidylcholines / metabolism*
Phospholipids / metabolism
Tumor Cells, Cultured / drug effects,  metabolism
Grant Support
ID/Acronym/Agency:
R01CA57212/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Lipids; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids

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